Caspase 10 Antibody - #DF6021

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产品: Caspase 10 抗体
货号: DF6021
描述: Rabbit polyclonal antibody to Caspase 10
应用: WB IHC
反应: Human, Mouse, Rat
分子量: 59kDa; 59kD(Calculated).
蛋白号: Q92851
RRID: AB_2837996

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Caspase 10 Antibody detects endogenous levels of total Caspase 10.
RRID:
AB_2837996
引用格式: Affinity Biosciences Cat# DF6021, RRID:AB_2837996.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ALPS2; Apoptotic protease Mch-4; CASP 10; CASP-10; CASP10; CASPA_HUMAN; Caspase 10 apoptosis related cysteine peptidase; Caspase-10 subunit p12; FADD like ICE2; Fas associated death domain protein; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE 2; FLICE2; ICE like apoptotic protease 4; ICE-like apoptotic protease 4; Interleukin 1B converting enzyme 2; MCH 4;

抗原和靶标

免疫原:
Uniprot:

Q92851(CASPA_HUMAN) >>Visit HPA database.

基因/基因ID:
CASP10(843)
表达:
Q92851 CASPA_HUMAN:

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

描述:
Caspase-10 is a DED (death effector domain)-containing caspase and functions as an initiator caspase in Fas/TNF induced apoptosis (1). Four isoforms of caspase-10 have been identified: caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d. They have the same prodomain but different mature large and small subdomains (2-4). Upon death ligand-receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction (1-4). This complex processes procaspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, leading to apoptosis (3-6).
序列:
MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCIGLVPNKKLEKSSSASDVFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERLLPTRQRVSLFRNLLYELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQGKIDEDNLTCLEDLCKTVVPKLLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQTDVKTFLEALPQESWQNKHAGSNGNRATNGAPSLVSRGMQGASANTLNSETSTKRAAVYRMNRNHRGLCVIVNNHSFTSLKDRQGTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGRFGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPEQAPTSLQDSIPAEADFLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRMLKFLEKTMEIRGRKRTVWGAKQISATSLPTAISAQTPRPPMRRWSSVS

翻译修饰 - Q92851 作为底物

Site PTM Type Enzyme
K2 Methylation
K2 Ubiquitination
S3 Phosphorylation
Y9 Phosphorylation
K14 Ubiquitination
K17 Ubiquitination
K23 Ubiquitination
K90 Ubiquitination
K99 Ubiquitination
K158 Ubiquitination
K161 Ubiquitination
K175 Ubiquitination
K180 Ubiquitination
K192 Ubiquitination
T198 Phosphorylation
S207 Phosphorylation
S216 Phosphorylation
K235 Ubiquitination
S250 Phosphorylation
S260 Phosphorylation
T263 Phosphorylation
T268 Phosphorylation
S269 Phosphorylation
K271 Ubiquitination
S293 Phosphorylation
K338 Ubiquitination

研究背景

功能:

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.

Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis.

Isoform C is proteolytically inactive.

翻译修饰:

Cleavage by granzyme B and autocatalytic activity generate the two active subunits.

组织特异性:

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

亚基结构:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit (By similarity). Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Interacts with RFFL and RNF34; negatively regulate CASP10 through proteasomal degradation. Interacts with RIOK3.

蛋白家族:

Belongs to the peptidase C14A family.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

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