| 产品: | IRS1 抗体 |
| 货号: | DF6048 |
| 描述: | Rabbit polyclonal antibody to IRS1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 132kDa; 132kD(Calculated). |
| 蛋白号: | P35568 |
| RRID: | AB_2838018 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6048, RRID:AB_2838018.
展开/折叠
HIRS 1; HIRS1; Insulin receptor substrate 1; IRS 1; IRS-1; IRS1; IRS1_HUMAN; OTTHUMP00000164234;
抗原和靶标
- P35568 IRS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASPPESDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKGHHDGAAALGAGGGGGSCSGSSGLGEAGEDLSYGDVPPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSNCSNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTHAHRHRGSARLHPPLNHSRSIPMPASRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGPSTLTAPNGHYILSRGGNGHRCTPGTGLGTSPALAGDEAASAADLDNRFRKRTHSAGTSPTITHQKTPSQSSVASIEEYTEMMPAYPPGGGSGGRLPGHRHSAFVPTRSYPEEGLEMHPLERRGGHHRPDSSTLHTDDGYMPMSPGVAPVPSGRKGSGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGGCSPDIGGGPSSSSSSSNAVPSGTSYGKLWTNGVGGHHSHVLPHPKPPVESSGGKLLPCTGDYMNMSPVGDSNTSSPSDCYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSTSSGRLLYAATADDSSSSTSSDSLGGGYCGARLEPSLPHPHHQVLQPHLPRKVDTAAQTNSRLARPTRLSLGDPKASTLPRAREQQQQQQPLLHPPEPKSPGEYVNIEFGSDQSGYLSGPVAFHSSPSVRCPSQLQPAPREEETGTEEYMKMDLGPGRRAAWQESTGVEMGRLGPAPPGAASICRPTRAVPSSRGDYMTMQMSCPRQSYVDTSPAAPVSYADMRTGIAAEEVSLPRATMAAASSSSAASASPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASFENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPPPPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P35568 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| S3 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | P17252 (PRKCA) , P53350 (PLK1) , P67870 (CSNK2B) | Uniprot |
| Y46 | Phosphorylation | Uniprot | |
| Y47 | Phosphorylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| K61 | Acetylation | Uniprot | |
| Y87 | Phosphorylation | Uniprot | |
| T88 | Phosphorylation | P67870 (CSNK2B) | Uniprot |
| S99 | Phosphorylation | P67870 (CSNK2B) | Uniprot |
| K169 | Ubiquitination | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S270 | Phosphorylation | Q13535 (ATR) , P23443 (RPS6KB1) , O14920 (IKBKB) | Uniprot |
| S272 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S273 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S303 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Q02750 (MAP2K1) , Q05655 (PRKCD) , P45984 (MAPK9) , P23443 (RPS6KB1) , O14920 (IKBKB) , P45983 (MAPK8) , P42345 (MTOR) | Uniprot |
| T309 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | P28482 (MAPK1) , O14733 (MAP2K7) , O14920 (IKBKB) , P45983 (MAPK8) | Uniprot |
| S315 | Phosphorylation | P45983 (MAPK8) , P45984 (MAPK9) | Uniprot |
| S323 | Phosphorylation | Q05655 (PRKCD) , P05771 (PRKCB) , Q05513 (PRKCZ) | Uniprot |
| S329 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | P67870 (CSNK2B) | Uniprot |
| T335 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S345 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S348 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| S372 | Phosphorylation | Uniprot | |
| R373 | Methylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| Y431 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Q05655 (PRKCD) , P05771 (PRKCB) , Q05513 (PRKCZ) | Uniprot |
| S444 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| Y465 | Phosphorylation | P08069 (IGF1R) | Uniprot |
| Y483 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| T495 | Phosphorylation | Uniprot | |
| T498 | Phosphorylation | Uniprot | |
| T502 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S503 | Phosphorylation | Uniprot | |
| T525 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | P23443 (RPS6KB1) , O14920 (IKBKB) | Uniprot |
| T530 | Phosphorylation | Uniprot | |
| S531 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| T533 | Phosphorylation | Uniprot | |
| S574 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| S581 | Phosphorylation | Uniprot | |
| S603 | Phosphorylation | Uniprot | |
| S604 | Phosphorylation | Uniprot | |
| T605 | Phosphorylation | Uniprot | |
| T608 | Phosphorylation | Uniprot | |
| Y612 | Phosphorylation | P23458 (JAK1) , P08069 (IGF1R) , P45984 (MAPK9) | Uniprot |
| S616 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8) , Q05655 (PRKCD) | Uniprot |
| S624 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | P31749 (AKT1) | Uniprot |
| Y632 | Phosphorylation | P45984 (MAPK9) , P23458 (JAK1) , P08069 (IGF1R) | Uniprot |
| S636 | Phosphorylation | P23443 (RPS6KB1) , O75116 (ROCK2) , P45983 (MAPK8) , P28482 (MAPK1) | Uniprot |
| S639 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| Y662 | Phosphorylation | P08069 (IGF1R) , P23458 (JAK1) | Uniprot |
| S666 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| S680 | Phosphorylation | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| Y695 | Phosphorylation | Uniprot | |
| Y732 | Phosphorylation | P23458 (JAK1) , P08069 (IGF1R) | Uniprot |
| S736 | Phosphorylation | Uniprot | |
| S766 | Phosphorylation | Uniprot | |
| K772 | Methylation | Uniprot | |
| K772 | Ubiquitination | Uniprot | |
| S794 | Phosphorylation | Q9H0K1 (SIK2) | Uniprot |
| T811 | Phosphorylation | P67870 (CSNK2B) | Uniprot |
| T847 | Phosphorylation | Uniprot | |
| T859 | Phosphorylation | Uniprot | |
| S862 | Phosphorylation | Uniprot | |
| K867 | Ubiquitination | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| Y896 | Phosphorylation | P06241 (FYN) , P08069 (IGF1R) , P06213 (INSR) | Uniprot |
| T936 | Phosphorylation | Uniprot | |
| T938 | Phosphorylation | Uniprot | |
| Y941 | Phosphorylation | P08069 (IGF1R) | Uniprot |
| S974 | Phosphorylation | O75116 (ROCK2) | Uniprot |
| S984 | O-Glycosylation | Uniprot | |
| S984 | Phosphorylation | Uniprot | |
| S985 | O-Glycosylation | Uniprot | |
| Y989 | Phosphorylation | P06213 (INSR) , P08069 (IGF1R) | Uniprot |
| S1005 | Phosphorylation | Uniprot | |
| S1011 | O-Glycosylation | Uniprot | |
| Y1012 | Phosphorylation | Uniprot | |
| S1025 | Phosphorylation | Uniprot | |
| S1037 | Phosphorylation | Uniprot | |
| S1041 | Phosphorylation | Uniprot | |
| S1043 | Phosphorylation | Uniprot | |
| T1045 | Phosphorylation | Uniprot | |
| S1078 | Phosphorylation | Uniprot | |
| S1084 | Phosphorylation | Uniprot | |
| S1100 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S1101 | Phosphorylation | P05129 (PRKCG) , Q05513 (PRKCZ) , Q04759 (PRKCQ) , Q05655 (PRKCD) , P23443 (RPS6KB1) | Uniprot |
| T1103 | Phosphorylation | Uniprot | |
| S1105 | Phosphorylation | Uniprot | |
| T1107 | Phosphorylation | Uniprot | |
| S1109 | Phosphorylation | Uniprot | |
| T1111 | Phosphorylation | Uniprot | |
| T1116 | Phosphorylation | Uniprot | |
| S1142 | Phosphorylation | Uniprot | |
| S1143 | Phosphorylation | Uniprot | |
| S1145 | Phosphorylation | Uniprot | |
| Y1179 | Phosphorylation | P06213 (INSR) , P08069 (IGF1R) , P06241 (FYN) | Uniprot |
| S1222 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S1223 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y1229 | Phosphorylation | P06213 (INSR) , P08069 (IGF1R) | Uniprot |
研究背景
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity). Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1. Phosphorylated on tyrosine residues in response to insulin. In skeletal muscles, dephosphorylated on Tyr-612 by TNS2 under anabolic conditions; dephosphorylation results in the proteasomal degradation of IRS1.
Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).
Interacts with UBTF and PIK3CA (By similarity). Interacts (via phosphorylated YXXM motifs) with PIK3R1 (By similarity). Interacts with ROCK1 and FER (By similarity). Interacts (via PH domain) with PHIP (By similarity). Interacts with GRB2 (By similarity). Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Interacts with ALK. Interacts with EIF2AK2/PKR (By similarity). Interacts with GKAP1 (By similarity). Interacts with DGKZ in the absence of insulin; insulin stimulation decreases this interaction (By similarity). Found in a ternary complex with DGKZ and PIP5K1A in the absence of insulin stimulation (By similarity).
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
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