产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6062, RRID:AB_2838031.
展开/折叠
AWD; AWD, drosophila, homolog of; GAAD; Granzyme A activated DNase; Granzyme A-activated DNase; GZMA activated DNase; Metastasis inhibition factor NM23; NB; NBS; NDK A; NDKA; NDKA_HUMAN; NDP kinase A; NDPK-A; NDPKA; NM23; NM23 long variant, included; nm23-H1; NM23-M1; NM23H1B, included; NME/NM23 nucleoside diphosphate kinase 1; Nme1; NME1-NME2 spliced read-through transcript, included; Non-metastatic cells 1, protein (NM23A) expressed in; Nonmetastatic cells 1, protein expressed in; Nonmetastatic protein 23; Nonmetastatic protein 23, homolog 1; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein;
抗原和靶标
Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation.
- P15531 NDKA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15531 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R6 | Methylation | Uniprot | |
| K12 | Acetylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| R18 | Methylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | P15531 (NME1) | Uniprot |
| K49 | Acetylation | Uniprot | |
| K49 | Sumoylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| K56 | Acetylation | Uniprot | |
| K56 | Sumoylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| R58 | Methylation | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| K85 | Acetylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| R88 | Methylation | Uniprot | |
| T94 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S99 | Phosphorylation | Uniprot | |
| K100 | Acetylation | Uniprot | |
| K100 | Methylation | Uniprot | |
| K100 | Sumoylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| H118 | Phosphorylation | P15531 (NME1) | Uniprot |
| S120 | Phosphorylation | P15531 (NME1) , P06493 (CDK1) | Uniprot |
| S122 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S125 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
翻译修饰 - P15531 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P15531 (NME1) | S44 | Uniprot |
| P15531-2 (NME1) | S69 | Uniprot |
| P15531 (NME1) | H118 | Uniprot |
| P15531 (NME1) | S120 | Uniprot |
| P15531-1 (NME1) | S122 | Uniprot |
| P15531-1 (NME1) | S125 | Uniprot |
| P15531-2 (NME1) | S145 | Uniprot |
| P15531-2 (NME1) | S147 | Uniprot |
| P15531-2 (NME1) | S150 | Uniprot |
| Q8IVT5 (KSR1) | S406 | Uniprot |
| Q969H4-2 (CNKSR1) | S385 | Uniprot |
研究背景
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Cytoplasm. Nucleus.
Note: Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.
Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation.
Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with PRUNE1. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Within this complex, interacts directly with SET. Also interacts with TREX1, but only following translocation to the nucleus.
Belongs to the NDK family.
研究领域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.
· Metabolism > Global and overview maps > Metabolic pathways.
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