CARD8 Antibody - #DF6092

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产品: CARD8 抗体
货号: DF6092
描述: Rabbit polyclonal antibody to CARD8
应用: WB IHC
反应: Human, Mouse
分子量: 49kDa; 49kD(Calculated).
蛋白号: Q9Y2G2
RRID: AB_2838060

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
CARD8 Antibody detects endogenous levels of total CARD8.
RRID:
AB_2838060
引用格式: Affinity Biosciences Cat# DF6092, RRID:AB_2838060.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Apoptotic protein NDPP 1; Apoptotic protein NDPP1; CARD 8; CARD inhibitor of NF kappa B activating ligand; CARD inhibitor of NF kappaB activating ligand; CARD inhibitor of NF kappaB activating ligands; CARD-inhibitor of NF-kappa-B-activating ligand; CARD8; CARD8_HUMAN; CARDINAL; Caspase recruitment domain containing protein 8; Caspase recruitment domain family member 8; Caspase recruitment domain protein 8; Caspase recruitment domain-containing protein 8; DACAR; Dakar; DKFZp779L0366; FLJ18119; FLJ18121; KIAA0955; MGC57162; NDPP 1; NDPP; NDPP1; TUCAN; Tumor up regulated CARD containing antagonist of CASP9; Tumor up regulated CARD containing antagonist of caspase 9; Tumor up-regulated CARD-containing antagonist of CASP9; tumor up-regulated CARD-containing antagonist of caspase nine; Tumor upregulated CARD containing antagonist of CASP9;

抗原和靶标

免疫原:
Uniprot:

Q9Y2G2(CARD8_HUMAN) >>Visit HPA database.

基因/基因ID:
CARD8(22900)
表达:
Q9Y2G2 CARD8_HUMAN:

High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow.

描述:
The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined. [provided by RefSeq]
序列:
MMRQRQSHYCSVLFLSVNYLGGTFPGDICSEENQIVSSYASKVCFEIEEDYKNRQFLGPEGNVDVELIDKSTNRYSVWFPTAGWYLWSATGLGFLVRDEVTVTIAFGSWSQHLALDLQHHEQWLVGGPLFDVTAEPEEAVAEIHLPHFISLQGEVDVSWFLVAHFKNEGMVLEHPARVEPFYAVLESPSFSLMGILLRIASGTRLSIPITSNTLIYYHPHPEDIKFHLYLVPSDALLTKAIDDEEDRFHGVRLQTSPPMEPLNFGSSYIVSNSANLKVMPKELKLSYRSPGEIQHFSKFYAGQMKEPIQLEITEKRHGTLVWDTEVKPVDLQLVAASAPPPFSGAAFVKENHRQLQARMGDLKGVLDDLQDNEVLTENEKELVEQEKTRQSKNEALLSMVEKKGDLALDVLFRSISERDPYLVSYLRQQNL

翻译修饰 - Q9Y2G2 作为底物

Site PTM Type Enzyme
K52 Ubiquitination
Y229 Phosphorylation
T238 Phosphorylation
K315 Ubiquitination

研究背景

功能:

Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow.

亚基结构:

May form homodimers. Interacts with NEMO and DRAL. Binds to caspase-1, CARD16/pseudo-ICE and CARD18/ICEBERG. Interacts with FNBP3 (By similarity). Interacts with NALP2 NACHT domain.

研究领域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). CARD8 polymorphism rs2043211 protects against noise-induced hearing loss by causing the dysfunction of CARD8 protein. Environmental science and pollution research international, 2021 (PubMed: 33067783) [IF=5.8]

Application: WB    Species: human    Sample: HEK293 cells

Fig. 2| CARD8 protein expression levels in the HEK293 cells transfected with plasmid of wild type and mutant. aRepresentative images of western blot analysis of CARD8 in HEK293 cells transfected with plasmid of wild type and mutant, respectively.

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