NTF3 Antibody - #DF6105

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产品: NTF3 抗体
货号: DF6105
描述: Rabbit polyclonal antibody to NTF3
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 29kDa; 29kD(Calculated).
蛋白号: P20783
RRID: AB_2838073

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 50ul RMB¥ 1250 现货
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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
NTF3 Antibody detects endogenous levels of total NTF3.
RRID:
AB_2838073
引用格式: Affinity Biosciences Cat# DF6105, RRID:AB_2838073.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; Neurotrophin 3 precursor; Neurotrophin-3; Neurotrophin3; NGF 2; NGF-2; NGF2; NT 3; NT-3; NT3; NTF 3; NTF3; NTF3_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P20783(NTF3_HUMAN) >>Visit HPA database.

基因/基因ID:
NTF3(4908)
表达:
P20783 NTF3_HUMAN:

Brain and peripheral tissues.

描述:
NT-3 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-4 (1). NT-3 is expressed in a number of cell types including neuronal cells, eosinophils, and melanocytes (1-3). NT-3 is required for the development of peripheral sensory neurons (4). NT-3 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-3 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkC. While all neurotrophins bind to the p75NTR receptor, NT-3 preferentially binds to TrkC and, with lesser affinity, TrkB (1).
序列:
MSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPERGGPAKSAFQPVIAMDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGSPVVANRTSRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P20783 作为底物

Site PTM Type Enzyme
Y59 Phosphorylation
Y99 Phosphorylation
S101 Phosphorylation
Y123 Phosphorylation
T222 Phosphorylation

研究背景

功能:

Seems to promote the survival of visceral and proprioceptive sensory neurons.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Brain and peripheral tissues.

蛋白家族:

Belongs to the NGF-beta family.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

文献引用

1). Electroacupuncture improves neuronal plasticity through the A2AR/cAMP/PKA signaling pathway in SNL rats. Neurochemistry International, 2021 (PubMed: 33577869) [IF=4.2]

Application: WB    Species: rat    Sample: spinal cord

Fig. 8. EA decreased the protein expression levels of NTF3, NTF4, Synaptophysin and Synapsin I in SNL rats, while SCH58261 reversed this effect. (A), (B), (C), (D), (E) Protein expression levels of NTF3, NTF4, Synaptophysin and Synapsin I in the spinal cord and quantification data of the expression of NTF3/GAPDH, NTF4/ GAPDH, Synaptophysin/GAPDH and Synapsin I/GAPDH in each group. n = 5. Columns represent the mean ± SD. $$p < 0.01 vs. the S group; #p < 0.05, ##p < 0.01 vs. the M group; &&p < 0.01 vs. the M + EA group.
2). Oxycodone regulates incision-induced activation of neurotrophic factors and receptors in an acute post-surgery pain rat model. Journal of Pain Research, 2018 (PubMed: 30464584) [IF=2.7]

Application: WB    Species: rat    Sample: spinal cord

Figure 3 |The altered expression of NTs in the spinal cord 24 hours after PI and the effects of oxycodone on NTs.Notes: (A, C, and E) The mRNA level of NGF, BDNF, and NT-3. (B, D, and F) The upper panels represent results of the Western blots. The lower panels show the semiquantitative analysis of NGF, BDNF, and NT-3 after normalization to the corresponding β-actin (*P<0.05, **P<0.01, ***P<0.001 vs control group; #P<0.05, ##P<0.01, ###P<0.001 vs PI, one-way ANOVA followed by Bonferroni’s multiple comparison post hoc test, n=3–4 rats per group). Each value represents mean±SEM. PI+OXY, postoperative oxycodone group; OXY+PI, preoperative oxycodone group.
3). Volatile Oil from Acorus gramineus Ameliorates the Injury Neurons in the Hippocampus of Amyloid Beta 1-42 Injected Mice. ANATOMICAL RECORD, 2019 (PubMed: 31443117)

Application: IHC    Species: mouse    Sample: hippocampus

Fig. 7. |The immunohistochemical observation of NT3 expression in the hippocampus of mice among all experimental groups. (A-A00) Control group, (B-B00) saline group, (C-C00) AD group; (D-D00) AD plus per 10 g body weight 2.5 μL volatile oil group, (E-E00) AD plus per 10 g body weight 5 μL volatile oil group. Scale bars = 50 μm in image A–E, 20 μm in A0-E0, 10 μm in A00-E00.

Application: WB    Species: mouse    Sample: hippocampus

Fig. 7. |The immunohistochemical observation of NT3 expression in the hippocampus of mice among all experimental groups. The western blot results showed the expression levels of NT3 protein (F). The histogram was plotted according to the comparison of the relative density for NT3 in all groups (G). *P < 0.05 AD group vs. control group; #P < 0.05 different doses of volatile oil groups vs. AD group.

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