PEBP1 Antibody - #DF6110

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(1)   (8)  
产品: PEBP1 抗体
货号: DF6110
描述: Rabbit polyclonal antibody to PEBP1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 21kDa; 21kD(Calculated).
蛋白号: P30086
RRID: AB_2838078

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(90%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
PEBP1 Antibody detects endogenous levels of total PEBP1.
RRID:
AB_2838078
引用格式: Affinity Biosciences Cat# DF6110, RRID:AB_2838078.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Epididymis luminal protein 210; Epididymis secretory protein Li 34; Epididymis secretory protein Li 96; HCNP; HCNPpp; HEL 210; HEL S 34; HEL S 96; Hippocampal cholinergic neurostimulating peptide; Neuropolypeptide h3; PBP; PEBP; PEBP 1; PEBP-1; Pebp1; PEBP1_HUMAN; Phosphatidylethanolamine binding protein; Phosphatidylethanolamine binding protein 1; Prostatic binding protein; Prostatic-binding protein; R kip; Raf kinase inhibitor protein; Raf kinase inhibitory protein; RKIP;

抗原和靶标

免疫原:
Uniprot:

P30086(PEBP1_HUMAN) >>Visit HPA database.

基因/基因ID:
PEBP1(5037)
描述:
Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-κB pathway, including NF-κB-inducing kinase (NIK), TAK1, IKKα and IKKβ (5). These interactions antagonize cytokine-induced activation of the NF-κB pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6).
序列:
MPVDLSKWSGPLSLQEVDEQPQHPLHVTYAGAAVDELGKVLTPTQVKNRPTSISWDGLDSGKLYTLVLTDPDAPSRKDPKYREWHHFLVVNMKGNDISSGTVLSDYVGSGPPKGTGLHRYVWLVYEQDRPLKCDEPILSNRSGDHRGKFKVASFRKKYELRAPVAGTCYQAEWDDYVPKLYEQLSGK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Dog
90
Zebrafish
90
Rabbit
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P30086 作为底物

Site PTM Type Enzyme
S6 Phosphorylation
K7 Ubiquitination
S9 Phosphorylation
S13 Phosphorylation
T28 Phosphorylation
K39 Acetylation
T42 Phosphorylation
K47 Ubiquitination
T51 Phosphorylation
S52 Phosphorylation
S54 Phosphorylation
S60 Phosphorylation
K62 Acetylation
Y64 Phosphorylation
T69 Phosphorylation
S75 Phosphorylation
K77 Acetylation
K80 Acetylation
Y81 Phosphorylation
S98 Phosphorylation
S99 Phosphorylation
S104 Phosphorylation
Y106 Phosphorylation
S109 Phosphorylation
K113 Ubiquitination
Y120 Phosphorylation
Y125 Phosphorylation
R129 Methylation
K132 Acetylation
K132 Ubiquitination
S153 Phosphorylation P17252 (PRKCA) , Q05655 (PRKCD) , P05129 (PRKCG) , P05771 (PRKCB) , Q05513 (PRKCZ)
C168 S-Nitrosylation
Y169 Phosphorylation
Y176 Phosphorylation
K179 Acetylation
K179 Ubiquitination
Y181 Phosphorylation
S185 Phosphorylation
K187 Ubiquitination

研究背景

功能:

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.

HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Has a tendency to form dimers by disulfide cross-linking (By similarity). Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Interacts with ALOX15; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade.

蛋白家族:

Belongs to the phosphatidylethanolamine-binding protein family.

文献引用

1). Locostatin Alleviates Liver Fibrosis Induced by Carbon Tetrachloride in Mice. DIGESTIVE DISEASES AND SCIENCES, 2019 (PubMed: 30874989) [IF=3.1]

Application: WB    Species: mouse    Sample: liver

Fig. 2| Locostatin reduced liver α-SMA expression in mice treated with CCl4. a Representative α-SMA IHC liver stained sections. b *P < 0.05 compared to the control group;. #P < 0.01 compared to the CCl4 group. c *P < 0.05 compared to the control group;. #P < 0.05 compared to the CCl4 group

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.