SMNDC1 Antibody - #DF6121

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产品: SMNDC1 抗体
货号: DF6121
描述: Rabbit polyclonal antibody to SMNDC1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 27kDa; 27kD(Calculated).
蛋白号: O75940
RRID: AB_2838088

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
SMNDC1 Antibody detects endogenous levels of total SMNDC1.
RRID:
AB_2838088
引用格式: Affinity Biosciences Cat# DF6121, RRID:AB_2838088.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

30 kDa splicing factor SMNrp; MGC106917; MGC112663; SMN related protein; SMN-related protein; smndc1; SMNR; SPF30; SPF30_HUMAN; Splicing factor 30, survival of motor neuron-related; Survival motor neuron domain containing 1; Survival motor neuron domain containing protein 1; Survival motor neuron domain-containing protein 1; Survival of motor neuron related splicing factor 30; Survival of motor neuron-related-splicing factor 30;

抗原和靶标

免疫原:
Uniprot:

O75940(SPF30_HUMAN) >>Visit HPA database.

基因/基因ID:
SMNDC1(10285)
表达:
O75940 SPF30_HUMAN:

Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas.

描述:
SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (Survival Motor Neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.
序列:
MSEDLAKQLASYKAQLQQVEAALSGNGENEDLLKLKKDLQEVIELTKDLLSTQPSETLASSDSFASTQPTHSWKVGDKCMAVWSEDGQCYEAEIEEIDEENGTAAITFAGYGNAEVTPLLNLKPVEEGRKAKEDSGNKPMSKKEMIAQQREYKKKKALKKAQRIKELEQEREDQKVKWQQFNNRAYSKNKKGQVKRSIFASPESVTGKVGVGTCGIADKPMTQYQDTSKYNVRHLMPQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
91
Chicken
88
Zebrafish
55
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75940 作为底物

Site PTM Type Enzyme
K13 Ubiquitination
K37 Ubiquitination
S51 Phosphorylation
T52 Phosphorylation
T57 Phosphorylation
S63 Phosphorylation
S66 Phosphorylation
T67 Phosphorylation
T70 Phosphorylation
K165 Ubiquitination
R184 Methylation
S201 Phosphorylation
S204 Phosphorylation
T206 Phosphorylation
T213 Phosphorylation
K219 Acetylation
K219 Ubiquitination
T222 Phosphorylation
Y224 Phosphorylation
T227 Phosphorylation
K229 Ubiquitination

研究背景

功能:

Necessary for spliceosome assembly. Overexpression causes apoptosis.

细胞定位:

Nucleus speckle. Nucleus>Cajal body.
Note: Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas.

亚基结构:

Associates with spliceosomes. Associates with U4/U5/U6 tri-snRNP and with U2 snRNP.

蛋白家族:

The Tudor domain mediates association with dimethylarginines, which are common in snRNP proteins.

Belongs to the SMN family.

研究领域

· Genetic Information Processing > Transcription > Spliceosome.

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