EEF1A1 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IF/ICC

1/2

Western blot analysis of extracts from B16F10 cells, using EEF1A1 Antibody.

产品:	EEF1A1 抗体
货号:	DF6156
描述:	Rabbit polyclonal antibody to EEF1A1
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量:	50kDa; 50kD(Calculated).
蛋白号:	P68104
RRID:	AB_2838123

浏览相似产品>>

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

联系销售

实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

EEF1A1 Antibody detects endogenous levels of total EEF1A1.

RRID:

AB_2838123
引用格式: Affinity Biosciences Cat# DF6156, RRID:AB_2838123.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

CCS 3; CCS3; Cervical cancer suppressor 3; chunp6927; CTCL tumor antigen; EE1A1; EEF 1; EEF1A; eEF1A-1; EEF1A1; EF-1-alpha-1; EF-Tu; EF1A; EF1a like protein; EF1A1_HUMAN; Elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; Eukaryotic translation elongation factor 1 alpha 1; Eukaryotic translation elongation factor 1 alpha 1 like 14; Glucocorticoid receptor AF 1 specific elongation factor; GRAF 1EF; HNGC:16303; ik:tdsubc_2a3; ik:tdsubc_2b3; LENG7; Leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; Prostate tumor inducing protein 1; PTI1; tdsubc_2a3; Translation elongation factor 1 alpha 1 like 14; wu:fa91c07; wu:fa94b03; wu:fi13b09; xx:tdsubc_2a3; xx:tdsubc_2b3;

抗原和靶标

免疫原:

Uniprot:

P68104(EF1A1_HUMAN) >>Visit HPA database.

基因/基因ID:

EEF1A1(1915)

表达:

P68104 EF1A1_HUMAN:

Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.

描述:

Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2,3).

序列:

P68104 EF1A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P68104 作为底物

P68104

Site	PTM Type	Enzyme	Source
G2	Methylation		Uniprot
S21	Phosphorylation	P15056 (BRAF)	Uniprot
T22	Phosphorylation		Uniprot
T23	Phosphorylation		Uniprot
T24	Phosphorylation		Uniprot
Y29	Phosphorylation		Uniprot
K30	Acetylation		Uniprot
K30	Methylation		Uniprot
K30	Ubiquitination		Uniprot
K36	Acetylation		Uniprot
K36	Methylation		Uniprot
K36	Ubiquitination		Uniprot
R37	Methylation		Uniprot
K41	Acetylation		Uniprot
K41	Sumoylation		Uniprot
K41	Ubiquitination		Uniprot
K44	Acetylation		Uniprot
K44	Ubiquitination		Uniprot
K51	Methylation		Uniprot
K51	Ubiquitination		Uniprot
K55	Acetylation		Uniprot
K55	Methylation		Uniprot
K55	Sumoylation		Uniprot
K55	Ubiquitination		Uniprot
Y56	Phosphorylation		Uniprot
K62	Methylation		Uniprot
K62	Ubiquitination		Uniprot
S76	Phosphorylation		Uniprot
K79	Acetylation		Uniprot
K79	Methylation		Uniprot
T82	Phosphorylation		Uniprot
K84	Acetylation		Uniprot
K84	Methylation		Uniprot
K84	Ubiquitination		Uniprot
Y85	Phosphorylation		Uniprot
Y86	Phosphorylation		Uniprot
T88	Phosphorylation	P15056 (BRAF)	Uniprot
R96	Methylation		Uniprot
T104	Phosphorylation		Uniprot
T106	Phosphorylation		Uniprot
C111	S-Nitrosylation		Uniprot
S128	Phosphorylation		Uniprot
K129	Ubiquitination		Uniprot
T133	Phosphorylation		Uniprot
R134	Methylation		Uniprot
Y141	Phosphorylation		Uniprot
T142	Phosphorylation		Uniprot
K146	Acetylation		Uniprot
K146	Ubiquitination		Uniprot
K154	Methylation		Uniprot
K154	Ubiquitination		Uniprot
S157	Phosphorylation		Uniprot
T158	Phosphorylation		Uniprot
Y162	Phosphorylation		Uniprot
S163	Phosphorylation		Uniprot
K165	Methylation		Uniprot
K165	Ubiquitination		Uniprot
R166	Methylation		Uniprot
Y167	Phosphorylation		Uniprot
K172	Acetylation		Uniprot
K172	Methylation		Uniprot
K172	Ubiquitination		Uniprot
S175	Phosphorylation		Uniprot
T176	Phosphorylation		Uniprot
Y177	Phosphorylation		Uniprot
K179	Acetylation		Uniprot
K179	Methylation		Uniprot
K179	Ubiquitination		Uniprot
K180	Acetylation		Uniprot
K180	Sumoylation		Uniprot
K180	Ubiquitination		Uniprot
K212	Acetylation		Uniprot
K212	Sumoylation		Uniprot
K212	Ubiquitination		Uniprot
K215	Ubiquitination		Uniprot
K219	Ubiquitination		Uniprot
S224	Phosphorylation		Uniprot
C234	S-Nitrosylation		Uniprot
T239	Phosphorylation		Uniprot
T242	Phosphorylation		Uniprot
K244	Acetylation		Uniprot
K244	Ubiquitination		Uniprot
Y254	Phosphorylation		Uniprot
K255	Acetylation		Uniprot
K255	Methylation		Uniprot
K255	Ubiquitination		Uniprot
T261	Phosphorylation		Uniprot
R266	Methylation		Uniprot
T269	Phosphorylation		Uniprot
K273	Acetylation		Uniprot
K273	Ubiquitination		Uniprot
T286	Phosphorylation		Uniprot
K290	Ubiquitination		Uniprot
S291	Phosphorylation		Uniprot
S300	Phosphorylation	P36897 (TGFBR1)	Uniprot
K313	Sumoylation		Uniprot
K313	Ubiquitination		Uniprot
S316	Phosphorylation		Uniprot
K318	Acetylation		Uniprot
K318	Methylation		Uniprot
K318	Ubiquitination		Uniprot
S354	Phosphorylation		Uniprot
Y357	Phosphorylation		Uniprot
T365	Phosphorylation		Uniprot
S383	Phosphorylation		Uniprot
K385	Ubiquitination		Uniprot
K386	Acetylation		Uniprot
K386	Ubiquitination		Uniprot
K392	Acetylation		Uniprot
K392	Sumoylation		Uniprot
K392	Ubiquitination		Uniprot
K395	Acetylation		Uniprot
K395	Ubiquitination		Uniprot
S396	Phosphorylation		Uniprot
K408	Acetylation		Uniprot
K408	Methylation		Uniprot
K408	Ubiquitination		Uniprot
C411	S-Nitrosylation		Uniprot
S414	Phosphorylation		Uniprot
S416	Phosphorylation		Uniprot
Y418	Phosphorylation		Uniprot
R430	Methylation		Uniprot
T432	Phosphorylation	Q96RG2 (PASK) , O75116 (ROCK2)	Uniprot
K439	Acetylation		Uniprot
K439	Ubiquitination		Uniprot
K444	Ubiquitination		Uniprot
K450	Acetylation		Uniprot
K450	Ubiquitination		Uniprot
T452	Phosphorylation		Uniprot
K453	Acetylation		Uniprot
K453	Ubiquitination		Uniprot
S454	Phosphorylation		Uniprot
K457	Acetylation		Uniprot
K460	Acetylation		Uniprot

研究背景

P68104_EF1A1_HUMAN

功能:

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

翻译修饰:

ISGylated.

Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2.

Trimethylated at Lys-79 by EEF1AKMT1. Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation. Trimethylated at Lys-318 by EEF1AKMT2. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at Gly-2 by EEF1AKNMT. Mono- and dimethylated at Lys-55 by EEF1AKNMT; dimethylated form is predominant.

细胞定位:

Cytoplasm. Nucleus. Nucleus>Nucleolus. Cell membrane.
Note: Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340). Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580). Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934).

组织特异性:

Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.

亚基结构:

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1, TXK, XPO5 and KARS1. May interact with ERGIC2. Interacts with IFIT1 (via TPR repeats 4-7) (By similarity). Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation. Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner. Interacts with PPP1R16B. Interacts with SPHK1 and SPHK2; both interactions increase SPHK1 and SPHK2 kinase activity.

蛋白家族:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

研究领域

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.

EEF1A1 Antibody - #DF6156

产品推荐

相关下载

MS Report

HPLC Report

MSDS

说明书

COA