MSH6 Antibody | Affinity Biosciences LTD|亲科生物官网

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Western blot analysis of Hela whole cell lysates, using MSH6 Antibody.

产品:	MSH6 抗体
货号:	DF6165
描述:	Rabbit polyclonal antibody to MSH6
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量:	153kDa; 153kD(Calculated).
蛋白号:	P52701
RRID:	AB_2838132

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(83%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(82%), Dog(91%)

克隆:

Polyclonal

特异性:

MSH6 Antibody detects endogenous levels of total MSH6.

RRID:

AB_2838132
引用格式: Affinity Biosciences Cat# DF6165, RRID:AB_2838132.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

DNA mismatch repair protein Msh6; G/T mismatch binding protein; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC 5; HNPCC5; HSAP; MSH 6; MSH6; MSH6_HUMAN; mutS (E. coli) homolog 6; MutS alpha 160 kDa subunit; MutS homolog 6 (E. coli); mutS homolog 6; MutS-alpha 160 kDa subunit; p160; Sperm associated protein;

抗原和靶标

免疫原:

Uniprot:

P52701(MSH6_HUMAN) >>Visit HPA database.

基因/基因ID:

MSH6(2956)

描述:

The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4).

序列:

P52701 MSH6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPEDFLNSCTPGMRKWWQIKSQNFDLVICYKVGKFYELYHMDALIGVSELGLVFMKGNWAHSGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMAHISKYDRVVRREICRIITKGTQTYSVLEGDPSENYSKYLLSLKEKEEDSSGHTRAYGVCFVDTSLGKFFIGQFSDDRHCSRFRTLVAHYPPVQVLFEKGNLSKETKTILKSSLSCSLQEGLIPGSQFWDASKTLRTLLEEEYFREKLSDGIGVMLPQVLKGMTSESDSIGLTPGEKSELALSALGGCVFYLKKCLIDQELLSMANFEEYIPLDSDTVSTTRSGAIFTKAYQRMVLDAVTLNNLEIFLNGTNGSTEGTLLERVDTCHTPFGKRLLKQWLCAPLCNHYAINDRLDAIEDLMVVPDKISEVVELLKKLPDLERLLSKIHNVGSPLKSQNHPDSRAIMYEETTYSKKKIIDFLSALEGFKVMCKIIGIMEEVADGFKSKILKQVISLQTKNPEGRFPDLTVELNRWDTAFDHEKARKTGLITPKAGFDSDYDQALADIRENEQSLLEYLEKQRNRIGCRTIVYWGIGRNRYQLEIPENFTTRNLPEEYELKSTKKGCKRYWTKTIEKKLANLINAEERRDVSLKDCMRRLFYNFDKNYKDWQSAVECIAVLDVLLCLANYSRGGDGPMCRPVILLPEDTPPFLELKGSRHPCITKTFFGDDFIPNDILIGCEEEEQENGKAYCVLVTGPNMGGKSTLMRQAGLLAVMAQMGCYVPAEVCRLTPIDRVFTRLGASDRIMSGESTFFVELSETASILMHATAHSLVLVDELGRGTATFDGTAIANAVVKELAETIKCRTLFSTHYHSLVEDYSQNVAVRLGHMACMVENECEDPSQETITFLYKFIKGACPKSYGFNAARLANLPEEVIQKGHRKAREFEKMNQSLRLFREVCLASERSTVDAEAVHKLLTLIKEL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Horse

100

Bovine

Sheep

Dog

Pig

Rabbit

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P52701 作为底物

P52701

Site	PTM Type	Source
Y8	Phosphorylation	Uniprot
S9	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
K22	Ubiquitination	Uniprot
R33	Methylation	Uniprot
S41	Phosphorylation	Uniprot
S43	Phosphorylation	Uniprot
S51	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
K70	Acetylation	Uniprot
S79	Phosphorylation	Uniprot
T86	Phosphorylation	Uniprot
S87	Phosphorylation	Uniprot
S91	Phosphorylation	Uniprot
S137	Phosphorylation	Uniprot
T139	Phosphorylation	Uniprot
R140	Methylation	Uniprot
S144	Phosphorylation	Uniprot
Y151	Phosphorylation	Uniprot
K155	Acetylation	Uniprot
K161	Ubiquitination	Uniprot
K169	Sumoylation	Uniprot
K169	Ubiquitination	Uniprot
S200	Phosphorylation	Uniprot
T212	Phosphorylation	Uniprot
T213	Phosphorylation	Uniprot
Y214	Phosphorylation	Uniprot
T216	Phosphorylation	Uniprot
S219	Phosphorylation	Uniprot
S227	Phosphorylation	Uniprot
T235	Phosphorylation	Uniprot
S252	Phosphorylation	Uniprot
S254	Phosphorylation	Uniprot
S256	Phosphorylation	Uniprot
S261	Phosphorylation	Uniprot
T269	Phosphorylation	Uniprot
S274	Phosphorylation	Uniprot
S275	Phosphorylation	Uniprot
S279	Phosphorylation	Uniprot
S280	Phosphorylation	Uniprot
S285	Phosphorylation	Uniprot
S292	Phosphorylation	Uniprot
T305	Phosphorylation	Uniprot
S309	Phosphorylation	Uniprot
K324	Ubiquitination	Uniprot
K334	Sumoylation	Uniprot
K334	Ubiquitination	Uniprot
S348	Phosphorylation	Uniprot
K374	Ubiquitination	Uniprot
K476	Ubiquitination	Uniprot
K479	Ubiquitination	Uniprot
T488	Phosphorylation	Uniprot
K498	Ubiquitination	Uniprot
K504	Acetylation	Uniprot
K504	Ubiquitination	Uniprot
K519	Ubiquitination	Uniprot
K537	Ubiquitination	Uniprot
K543	Ubiquitination	Uniprot
R577	Methylation	Uniprot
K598	Ubiquitination	Uniprot
K603	Ubiquitination	Uniprot
K610	Ubiquitination	Uniprot
K632	Ubiquitination	Uniprot
Y642	Phosphorylation	Uniprot
K646	Ubiquitination	Uniprot
K660	Ubiquitination	Uniprot
S668	Phosphorylation	Uniprot
K693	Ubiquitination	Uniprot
S702	Phosphorylation	Uniprot
Y709	Phosphorylation	Uniprot
K728	Ubiquitination	Uniprot
T767	Phosphorylation	Uniprot
K771	Ubiquitination	Uniprot
K813	Ubiquitination	Uniprot
K814	Ubiquitination	Uniprot
K824	Ubiquitination	Uniprot
S830	Phosphorylation	Uniprot
K833	Methylation	Uniprot
K833	Ubiquitination	Uniprot
K852	Ubiquitination	Uniprot
K854	Ubiquitination	Uniprot
K866	Ubiquitination	Uniprot
K870	Ubiquitination	Uniprot
K883	Ubiquitination	Uniprot
K888	Ubiquitination	Uniprot
T895	Phosphorylation	Uniprot
K896	Ubiquitination	Uniprot
T914	Phosphorylation	Uniprot
K920	Ubiquitination	Uniprot
K923	Ubiquitination	Uniprot
T924	Phosphorylation	Uniprot
T928	Phosphorylation	Uniprot
K930	Ubiquitination	Uniprot
S935	Phosphorylation	Uniprot
K957	Ubiquitination	Uniprot
K997	Sumoylation	Uniprot
K997	Ubiquitination	Uniprot
K1009	Ubiquitination	Uniprot
T1010	Phosphorylation	Uniprot
K1014	Ubiquitination	Uniprot
K1030	Ubiquitination	Uniprot
T1085	Phosphorylation	Uniprot
K1092	Methylation	Uniprot
K1092	Ubiquitination	Uniprot
K1101	Ubiquitination	Uniprot
K1126	Methylation	Uniprot
K1140	Ubiquitination	Uniprot
R1172	Methylation	Uniprot
K1240	Ubiquitination	Uniprot
K1291	Ubiquitination	Uniprot
K1296	Ubiquitination	Uniprot
K1315	Ubiquitination	Uniprot
K1325	Ubiquitination	Uniprot
K1352	Ubiquitination	Uniprot
K1358	Ubiquitination	Uniprot

研究背景

P52701_MSH6_HUMAN

功能:

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

翻译修饰:

The N-terminus is blocked.

Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

细胞定位:

Nucleus. Chromosome.
Note: Associates with H3K36me3 via its PWWP domain.

亚基结构:

Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with MCM9. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains.

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

蛋白家族:

The PWWP domain specifically recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3).

Belongs to the DNA mismatch repair MutS family.

研究领域

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

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MSH6 Antibody - #DF6165

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