产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6181, RRID:AB_2838147.
展开/折叠
Cytoplasmic protein NCK1; Melanoma Nck protein; MGC12668; NCK 1; NCK adaptor protein 1; NCK alpha; NCK; NCK tyrosine kinase; Nck-1; NCK1; NCK1_HUMAN; NCKalpha; Non catalytic region of tyrosine kinase; SH2/SH3 adaptor protein NCK alpha; SH2/SH3 adaptor protein NCK-alpha;
抗原和靶标
- P16333 NCK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P16333 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | P00519 (ABL1) | Uniprot |
| Y112 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| T161 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| Y268 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| Y339 | Phosphorylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot |
研究背景
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.
Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.
Cytoplasm. Endoplasmic reticulum. Nucleus.
Note: Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
Interacts (via SH2 domain and SH3 domain 2) with EGFR. Interacts with PAK1 and SOS1. Interacts (via SH3 domains) with PKN2. Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7. This interaction is required for nuclear import. Part of a complex containing PPP1R15B, PP1 and NCK1. Interacts with RALGPS1. Interacts with CAV2 (tyrosine phosphorylated form). Interacts with ADAM15. Interacts with FASLG. Directly interacts with RASA1. Interacts with isoform 4 of MINK1. Interacts with FLT1 (tyrosine phosphorylated). Interacts with KDR (tyrosine phosphorylated). Interacts (via SH2 domain) with EPHB1; activates the JUN cascade to regulate cell adhesion. Interacts with EPHA2. Interacts (via SH2 domain) with PDGFRB (tyrosine phosphorylated). Interacts with the inactive form of EIF2AK2/PKR.
Only the first and third SH3 domains seem to be involved in RASA1-binding; the second SH3 domain and the SH2 domains do not seem to be involved.
研究领域
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
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