| 产品: | TPMT 抗体 |
| 货号: | DF6183 |
| 描述: | Rabbit polyclonal antibody to TPMT |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Rabbit |
| 分子量: | 28kDa, 35 kDa; 28kD(Calculated). |
| 蛋白号: | P51580 |
| RRID: | AB_2838149 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6183, RRID:AB_2838149.
展开/折叠
HGNC:12014; S adenosyl L methionine thiopurine S methyltransferase; Thiopurine methyltransferase; Thiopurine S methyltransferase; Thiopurine S-methyltransferase; TPMT; TPMT_HUMAN;
抗原和靶标
- P51580 TPMT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRCLEKVDAFEERHKSWGIDCLFEKLYLLTEK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P51580 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T6 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| T16 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| K32 | Acetylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K58 | Acetylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K77 | Acetylation | Uniprot | |
| Y166 | Phosphorylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot |
研究背景
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor. TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.
Cytoplasm.
Monomer.
Belongs to the class I-like SAM-binding methyltransferase superfamily. TPMT family.
研究领域
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.
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