| 产品: | ADA 抗体 |
| 货号: | DF6184 |
| 描述: | Rabbit polyclonal antibody to ADA |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 41kDa; 41kD(Calculated). |
| 蛋白号: | P00813 |
| RRID: | AB_2838150 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6184, RRID:AB_2838150.
展开/折叠
ada; ADA_HUMAN; ADA1; Adenosine aminohydrolase; Adenosine deaminase;
抗原和靶标
Found in all tissues, occurs in large amounts in T-lymphocytes (PubMed:20959412). Expressed at the time of weaning in gastrointestinal tissues.
- P00813 ADA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00813 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T4 | Phosphorylation | Uniprot | |
| K9 | Acetylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| Y29 | Phosphorylation | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| K54 | Acetylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| Y84 | Phosphorylation | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K164 | Acetylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K170 | Acetylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K232 | Acetylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| Y249 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| Y290 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| K312 | Acetylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K323 | Acetylation | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| Y348 | Phosphorylation | Uniprot | |
| Y351 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot |
研究背景
Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development (By similarity).
Cell membrane>Peripheral membrane protein>Extracellular side. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Lysosome.
Note: Colocalized with DPP4 at the cell surface.
Found in all tissues, occurs in large amounts in T-lymphocytes. Expressed at the time of weaning in gastrointestinal tissues.
Interacts with DPP4 (via extracellular domain). Interacts with PLG (via Kringle 4 domain); the interaction stimulates PLG activation when in complex with DPP4.
Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.
研究领域
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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