SERPINA3 Antibody - #DF6185

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(1)   (4)  
产品: SERPINA3 抗体
货号: DF6185
描述: Rabbit polyclonal antibody to SERPINA3
应用: WB IHC
反应: Human, Mouse, Rat
预测: Rabbit
分子量: 48kDa; 48kD(Calculated).
蛋白号: P01011
RRID: AB_2838151

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Rabbit(80%)
克隆:
Polyclonal
特异性:
SERPINA3 Antibody detects endogenous levels of total SERPINA3.
RRID:
AB_2838151
引用格式: Affinity Biosciences Cat# DF6185, RRID:AB_2838151.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

SERPINA3; AACT; AACT_HUMAN; ACT; Alpha-1-antichymotrypsin His-Pro-less; Antichymotrypsin; Cell growth inhibiting gene 24/25 protein; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; Growth inhibiting protein 24; Growth inhibiting protein 25; MGC88254; Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3; Serine (or cysteine) proteinase inhibitor clade A member 3; Serine proteinase inhibitor clade A member 3; Serpin A3; Serpin family A member 3; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3; SERPINA3;

抗原和靶标

免疫原:
Uniprot:

P01011(AACT_HUMAN) >>Visit HPA database.

基因/基因ID:
SERPINA3(12)
表达:
P01011 AACT_HUMAN:

Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

描述:
The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq, Jul 2008]
序列:
MERMLPLLALGLLAAGFCPAVLCHPNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
80
Horse
70
Dog
70
Xenopus
67
Bovine
44
Pig
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P01011 作为底物

Site PTM Type Enzyme
N33 N-Glycosylation
T35 Phosphorylation
N93 N-Glycosylation
N106 N-Glycosylation
N127 N-Glycosylation
K158 Acetylation
K177 Acetylation
N186 N-Glycosylation
K191 Acetylation
S201 Phosphorylation
T203 Phosphorylation
K235 Acetylation
K236 Acetylation
K237 Acetylation
C261 S-Nitrosylation
N271 N-Glycosylation
S273 Phosphorylation
T295 Phosphorylation
S302 Phosphorylation
T338 Phosphorylation
T347 O-Glycosylation
T347 Phosphorylation
S384 Phosphorylation
K416 Acetylation
K421 Acetylation

研究背景

功能:

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

翻译修饰:

N- and O-glycosylated.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

亚基结构:

Interacts with DNAJC1.

蛋白家族:

The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

Belongs to the serpin family.

文献引用

1). Serpina3n/serpina3 alleviates cyclophosphamide-induced interstitial cystitis by activating the Wnt/β-catenin signal. International Urology and Nephrology, 2023 (PubMed: 37594700) [IF=2.0]

Application: WB    Species: Human    Sample:

Fig. 1 Serpina3/serpina3n expression was down-regulated in IC/BPS. A Assessment of the pathological changes in human bladder tissues by HE staining (× 200). B Evaluation of mast-cell infiltration in human bladder tissues using toluidine blue staining (× 200). C, D The mRNA and protein levels of serpina3 in the bladder tissues from IC/BPS patients and healthy individuals were tested by qPCR and western blotting. E Assessment of the pathological changes in mouse bladder tissues by HE staining (× 200). F Evaluation of mast-cell infiltration in mouse bladder tissues using toluidine blue staining (× 200). G, H The mRNA and protein levels of serpina3 in the bladder tissues from IC/BPS patients and healthy individuals were tested by qPCR and western blotting. (*P 

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.