STAR Antibody - #DF6192

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产品: STAR 抗体
货号: DF6192
描述: Rabbit polyclonal antibody to STAR
应用: WB IHC
反应: Human, Mouse, Rat, Duck
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 32kDa; 32kD(Calculated).
蛋白号: P49675
RRID: AB_2838158

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Duck
预测:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
克隆:
Polyclonal
特异性:
STAR Antibody detects endogenous levels of total STAR.
RRID:
AB_2838158
引用格式: Affinity Biosciences Cat# DF6192, RRID:AB_2838158.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cholesterol trafficker; Luteinizing hormone induced protein; mitochondrial; Mitochondrial steroid acute regulatory protein; StAR; StAR related lipid transfer (START) domain containing 1; STAR_HUMAN; StARD1; START domain containing 1; START domain containing protein 1; START domain-containing protein 1; Steroid acute regulatory protein; Steroidogenic acute regulator; Steroidogenic acute regulatory protein; Steroidogenic acute regulatory protein mitochondrial;

抗原和靶标

免疫原:
Uniprot:

P49675(STAR_HUMAN) >>Visit HPA database.

基因/基因ID:
STAR(6770)
表达:
P49675 STAR_HUMAN:

Expressed in gonads, adrenal cortex and kidney.

描述:
Steroidogenic acute regulatory protein (StAR) plays a significant role in cholesterol transport from the cytoplasmic outer membrane to the inner mitochondrial membrane (1). The 37 kDa precursor is cleaved to generate an active 28 kDa protein capable of facilitating cholesterol metabolism into pregnenolone (2,3). StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue (3). Abnormalities in StAR gene expression are impacted in autosomal Lipoid Congenial Adrenal Hyperplasia (LCAH) resulting in defects in pregnenolone and cortisol synthesis (4). The mechanism of cholesterol binding to StAR has yet to be elucidated (4).
序列:
MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
89
Zebrafish
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P49675 作为底物

Site PTM Type Enzyme
S56 Phosphorylation Q13237 (PRKG2)
S57 Phosphorylation Q13237 (PRKG2)
S61 Phosphorylation
S100 Phosphorylation Q13237 (PRKG2)
S195 Phosphorylation
T204 Phosphorylation
S233 Phosphorylation P27361 (MAPK3)
K273 Acetylation

研究背景

功能:

Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.

细胞定位:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in gonads, adrenal cortex and kidney.

亚基结构:

May interact with TSPO.

研究领域

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Cholesterol metabolism.

文献引用

1). Naringenin improves ovarian health by reducing the serum androgen and eliminating follicular cysts in letrozole-induced polycystic ovary syndrome in the Sprague Dawley rats. Phytotherapy research : PTR, 2023 (PubMed: 37165686) [IF=7.2]
2). The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Frontiers in Pharmacology, 2020 (PubMed: 32457626) [IF=5.6]

Application: WB    Species: mouse    Sample: LC540 (17b-HSD3) cells

FIGURE 3 | Effects of H10 on 17b-HSD3 in LC540 (17b-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17b-HSD3 mRNA.(D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 mM, while the concentration of 3858858 (positive control) was 0.25 mM.
3). Effects of stocking density on ovarian development and maturation during the rearing period in Shan-ma ducks. POULTRY SCIENCE, 2022 (PubMed: 35358924) [IF=4.4]

Application: WB    Species: Shan-maducks    Sample: ovarian

Figure 5.| Effects of stocking densities on protein levels of the ovarian steroid biosynthesis pathway. The protein expression levels of CYP19A1,3b-HSD, and StAR in ovarian tissue. Data are presented as the mean § SEM, n = 6. Adjacent letters indicate significant differences (P < 0.05) and interphase letters indicate extremely significant differences (P < 0.01).
4). Suppression of Notch Signaling Stimulates Progesterone Synthesis by Enhancing the Expression of NR5A2 and NR2F2 in Porcine Granulosa Cells. Genes, 2020 (PubMed: 31978970) [IF=3.5]

Application: WB    Species: porcine    Sample: pGCs

Figure 2.| Effects of DAPT treatment on the expression of proteins involved in steroidogenesis in pGCs. (A) The gene expression of factors involved in cholesterol uptake (LDLR, VLDLR, SRB1, NPC1,SCP2) and progesterone synthesis (StAR, Cyp11a1, HSD3B); (B,C) The protein expression of StAR,Cyp11a1, and HSD3B

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