U2AF1 Antibody - #DF6195
产品: | U2AF1 抗体 |
货号: | DF6195 |
描述: | Rabbit polyclonal antibody to U2AF1 |
应用: | WB IHC |
反应: | Human, Mouse, Rat |
预测: | Zebrafish, Bovine, Dog, Chicken, Xenopus |
分子量: | 28kDa; 28kD(Calculated). |
蛋白号: | Q01081 |
RRID: | AB_2838161 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6195, RRID:AB_2838161.
展开/折叠
DKFZp313J1712; FP793; Human U2 snRNP auxiliary factor small subunit9; RN; RNU2AF1; Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor 1; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2AF1; U2AF1_HUMAN; U2AF35; U2AFBP;
抗原和靶标
- Q01081 U2AF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQSADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGGRERDRRRSRDRERSGRF
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q01081 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Y4 | Phosphorylation | Uniprot | |
S7 | Phosphorylation | Uniprot | |
T11 | Phosphorylation | Uniprot | |
K13 | Acetylation | Uniprot | |
K13 | Ubiquitination | Uniprot | |
K15 | Sumoylation | Uniprot | |
K15 | Ubiquitination | Uniprot | |
S19 | Phosphorylation | Uniprot | |
K39 | Methylation | Uniprot | |
S58 | Phosphorylation | Uniprot | |
S59 | Phosphorylation | Uniprot | |
S61 | Phosphorylation | Uniprot | |
C67 | S-Nitrosylation | Uniprot | |
S70 | Phosphorylation | Uniprot | |
T87 | Phosphorylation | Uniprot | |
K92 | Sumoylation | Uniprot | |
Y93 | Phosphorylation | Uniprot | |
C102 | S-Nitrosylation | Uniprot | |
K116 | Ubiquitination | Uniprot | |
K125 | Ubiquitination | Uniprot | |
S145 | Phosphorylation | Uniprot | |
R165 | Methylation | Uniprot | |
K175 | Acetylation | Uniprot | |
K175 | Methylation | Uniprot | |
K175 | Sumoylation | Uniprot | |
K175 | Ubiquitination | Uniprot | |
S178 | Phosphorylation | Uniprot | |
R179 | Methylation | Uniprot | |
S204 | Phosphorylation | Uniprot | |
S206 | Phosphorylation | Uniprot | |
R209 | Methylation | Uniprot | |
R211 | Methylation | Uniprot | |
S231 | Phosphorylation | Uniprot | |
S237 | Phosphorylation | Uniprot |
研究背景
Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Nucleus. Nucleus speckle.
Interacts (via RS domain) with PHF5A (via N-terminus) (By similarity). Identified in the spliceosome C complex. Heterodimer with U2AF2. Interacts with ZRANB2.
The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.
Belongs to the splicing factor SR family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
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