产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6206, RRID:AB_2838172.
展开/折叠
26S protease subunit S5a; 26S proteasome non ATPase regulatory subunit 4; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit rpn10; 26S proteasome regulatory subunit S5A; AF 1; AF; AF1; Angiocidin; Antisecretory factor 1; ASF; DS5a; MCB 1; MCB1; Multiubiquitin chain binding protein; Multiubiquitin chain-binding protein; OTTHUMP00000059963; Prosome macropain; Proteasome (prosome macropain) 26S subunit non ATPase 4; Proteasome 26S non ATPase subunit 4; Proteasome 26S subunit non ATPase 4; PSMD 4; Psmd4; PSMD4_HUMAN; pUB R5; pUBR5; Rpn 10; Rpn10; RPN10 homolog; S5A; S5a/antisecretory factor protein;
抗原和靶标
- P55036 PSMD4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P55036 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| C9 | S-Nitrosylation | Uniprot | |
| C37 | S-Nitrosylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| C87 | S-Nitrosylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| K122 | Acetylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| Y326 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| K365 | Acetylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| K368 | Acetylation | Uniprot | |
| K369 | Ubiquitination | Uniprot |
研究背景
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD4. Interacts with NUB1. Interacts with SQSTM1. Interacts with UBQLN4. Interacts with UBE3A. Interacts with UBQLN1 (via ubiquitin-like domain). Interacts with DDI2.
The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
Belongs to the proteasome subunit S5A family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
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