产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6209, RRID:AB_2838175.
展开/折叠
Actin like protein 3; Actin related protein 3; ACTR3; ARP3 actin related protein 3 homolog (yeast);
抗原和靶标
- P61158 ARP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61158 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| C8 | S-Nitrosylation | Uniprot | |
| C12 | S-Nitrosylation | Uniprot | |
| Y16 | Phosphorylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K53 | Acetylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| Y100 | Phosphorylation | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| T113 | Phosphorylation | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| Y184 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| T201 | Phosphorylation | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| K240 | Acetylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K244 | Acetylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K251 | Acetylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K254 | Acetylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K264 | Sumoylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| Y400 | Phosphorylation | Uniprot | |
| S418 | Phosphorylation | Uniprot |
研究背景
ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). Plays a role in ciliogenesis.
Cytoplasm>Cytoskeleton. Cell projection. Nucleus.
Note: In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) (PubMed:19109554).
Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Interacts weakly with MEFV.
Belongs to the actin family. ARP3 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
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