产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6213, RRID:AB_2838179.
展开/折叠
AK1A1_HUMAN; Akr1a1; Alcohol dehydrogenase [NADP+]; Alcohol dehydrogenase; Aldehyde reductase; Aldo keto reductase family 1 member A1 (aldehyde reductase); Aldo-keto reductase family 1 member A1; ALDR1; ALR; ARM; DD3; MGC12529; MGC1380;
抗原和靶标
Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung.
- P14550 AK1A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASCVLLHTGQKMPLIGLGTWKSEPGQVKAAVKYALSVGYRHIDCAAIYGNEPEIGEALKEDVGPGKAVPREELFVTSKLWNTKHHPEDVEPALRKTLADLQLEYLDLYLMHWPYAFERGDNPFPKNADGTICYDSTHYKETWKALEALVAKGLVQALGLSNFNSRQIDDILSVASVRPAVLQVECHPYLAQNELIAHCQARGLEVTAYSPLGSSDRAWRDPDEPVLLEEPVVLALAEKYGRSPAQILLRWQVQRKVICIPKSITPSRILQNIKVFDFTFSPEEMKQLNALNKNWRYIVPMLTVDGKRVPRDAGHPLYPFNDPY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P14550 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| Y50 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| C134 | S-Nitrosylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| Y325 | Phosphorylation | Uniprot |
研究背景
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde. Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity). Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Displays no reductase activity towards retinoids (By similarity).
Cytoplasm>Cytosol. Apical cell membrane.
Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung.
Monomer.
Belongs to the aldo/keto reductase family.
研究领域
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.
· Metabolism > Lipid metabolism > Glycerolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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