GSS Antibody - #DF6214

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产品: GSS 抗体
货号: DF6214
描述: Rabbit polyclonal antibody to GSS
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 52kDa; 52kD(Calculated).
蛋白号: P48637
RRID: AB_2838180

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
GSS Antibody detects endogenous levels of total GSS.
RRID:
AB_2838180
引用格式: Affinity Biosciences Cat# DF6214, RRID:AB_2838180.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n; Glutathione synthase; Glutathione synthetase; GSH S; GSH synthetase; GSH-S; GSHB_HUMAN; GSHS; GSS; HEL-S-64p; HEL-S-88n; MGC14098; OTTHUMP00000030711;

抗原和靶标

免疫原:
Uniprot:

P48637(GSHB_HUMAN) >>Visit HPA database.

基因/基因ID:
GSS(2937)
描述:
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]
序列:
MATNWGSLLQDKQQLEELARQAVDRALAEGVLLRTSQEPTSSEVVSYAPFTLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAAFLEQTLSSTIKQDDFTARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPALKQIEINTISASFGGLASRTPAVHRHVLSVLSKTKEAGKILSNNPSKGLALGIAKAWELYGSPNALVLLIAQEKERNIFDQRAIENELLARNIHVIRRTFEDISEKGSLDQDRRLFVDGQEIAVVYFRDGYMPRQYSLQNWEARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEGDQAIAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Dog
92
Xenopus
92
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P48637 作为底物

Site PTM Type Enzyme
A2 Acetylation
K12 Ubiquitination
K108 Ubiquitination
K112 Ubiquitination
S137 Phosphorylation
K141 Ubiquitination
T147 Phosphorylation
S149 Phosphorylation
K178 Ubiquitination
S181 Phosphorylation
K186 Acetylation
K186 Ubiquitination
Y199 Phosphorylation
K213 Ubiquitination
R230 Methylation
T238 Phosphorylation
K245 Ubiquitination
R252 Methylation
Y270 Phosphorylation
K293 Ubiquitination
K306 Ubiquitination
K364 Ubiquitination
K384 Ubiquitination
K400 Ubiquitination
S415 Phosphorylation
K443 Ubiquitination
K452 Ubiquitination

研究背景

亚基结构:

Homodimer.

蛋白家族:

Belongs to the eukaryotic GSH synthase family.

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Reconstructing hepatic metabolic profile and glutathione-mediated metabolic fate of acrylamide. Environmental Pollution, 2023 (PubMed: 37673322) [IF=8.9]
2). Poria cocos polysaccharides improve alcoholic liver disease by interfering with ferroptosis through NRF2 regulation. Aging, 2024 (PubMed: 38507458) [IF=5.2]
3). DL-3-n-butylphthalide improves ventricular function, and prevents ventricular remodeling and arrhythmias in post-MI rats. Naunyn-Schmiedeberg's Archives of Pharmacology, 2018 (PubMed: 29602954) [IF=3.6]

Application: WB    Species: rat    Sample: ventricular

Fig. 6 |NBP upregulated PI3k/Akt/Nrf2 signaling pathway and downstream antioxidant enzymes, including HO-1, GSH, SOD1, and SOD2, analyzed by western blot. Data are expressed as means + SD. *P < 0.05 versus Sham group; #P < 0.05 versus MI group

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