产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6222, RRID:AB_2838188.
展开/折叠
ACSVL1; FACVL1; FATP 2; FATP-2; FATP2; Fatty acid coenzyme A ligase, very long chain 1; Fatty acid transport protein 2; Fatty-acid-coenzyme A ligase; hFACVL1; HsT17226; Long chain fatty acid CoA ligase; Long-chain-fatty-acid--CoA ligase; S27A2_HUMAN; Slc27a2; Solute carrier family 27 (fatty acid transporter), member 2; Solute carrier family 27 member 2; THCA CoA ligase; THCA-CoA ligase; Very long chain acyl CoA synthetase; Very long chain fatty acid CoA ligase; Very long chain fatty acid coenzyme A ligase 1; very long-chain 1; Very long-chain acyl-CoA synthetase; Very long-chain-fatty-acid-CoA ligase; VLACS; VLCS;
抗原和靶标
- O14975 S27A2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSAIYTVLAGLLFLPLLVNLCCPYFFQDIGYFLKVAAVGRRVRSYGKRRPARTILRAFLEKARQTPHKPFLLFRDETLTYAQVDRRSNQVARALHDHLGLRQGDCVALLMGNEPAYVWLWLGLVKLGCAMACLNYNIRAKSLLHCFQCCGAKVLLVSPELQAAVEEILPSLKKDDVSIYYVSRTSNTDGIDSFLDKVDEVSTEPIPESWRSEVTFSTPALYIYTSGTTGLPKAAMITHQRIWYGTGLTFVSGLKADDVIYITLPFYHSAALLIGIHGCIVAGATLALRTKFSASQFWDDCRKYNVTVIQYIGELLRYLCNSPQKPNDRDHKVRLALGNGLRGDVWRQFVKRFGDICIYEFYAATEGNIGFMNYARKVGAVGRVNYLQKKIITYDLIKYDVEKDEPVRDENGYCVRVPKGEVGLLVCKITQLTPFNGYAGAKAQTEKKKLRDVFKKGDLYFNSGDLLMVDHENFIYFHDRVGDTFRWKGENVATTEVADTVGLVDFVQEVNVYGVHVPDHEGRIGMASIKMKENHEFDGKKLFQHIADYLPSYARPRFLRIQDTIEITGTFKHRKMTLVEEGFNPAVIKDALYFLDDTAKMYVPMTEDIYNAISAKTLKL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14975 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S158 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K291 | Acetylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| Y304 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| K419 | Ubiquitination | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| K575 | Ubiquitination | Uniprot | |
| T577 | Phosphorylation | Uniprot | |
| K589 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| Y602 | Phosphorylation | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| K616 | Ubiquitination | Uniprot |
研究背景
Acyl CoA synthetase that activates long-chain and very long-chain fatty acids (VLCFAs) by catalyzing the formation of fatty acyl-CoA. Can also activate branched-chain fatty acids such as phytanic acid and pristanic acid. Does not activate C24 bile acids, cholate and chenodeoxycholate. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Exhibits long-chain fatty acids (LCFA) transport activity and plays an important role in hepatic fatty acid uptake.
Isoform 1 exhibits both long-chain fatty acids (LCFA) transport activity and acyl CoA synthetase towards very long-chain fatty acids. Shows a preference for generating CoA derivatives of n-3 fatty acids, which are preferentially trafficked into phosphatidylinositol.
Isoform 2 exhibits long-chain fatty acids (LCFA) transport activity but lacks acyl CoA synthetase towards very long-chain fatty acids.
Endoplasmic reticulum membrane>Multi-pass membrane protein. Peroxisome membrane>Peripheral membrane protein. Cell membrane>Multi-pass membrane protein. Microsome.
Expressed in liver, kidney, placenta and pancreas.
Belongs to the ATP-dependent AMP-binding enzyme family.
研究领域
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Organismal Systems > Endocrine system > PPAR signaling pathway.
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