PDIA3 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IF/ICC
Cites

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Western blot analysis of extracts from HepG2, using PDIA3 Antibody.

DF6230 staining Hela cells by IF/ICC.

Figure 4.

Figure 5.

产品:	PDIA3 抗体
货号:	DF6230
描述:	Rabbit polyclonal antibody to PDIA3
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量:	57kDa; 57kD(Calculated).
蛋白号:	P30101
RRID:	AB_2838196

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(83%)

克隆:

Polyclonal

特异性:

PDIA3 Antibody detects endogenous levels of total PDIA3.

RRID:

AB_2838196
引用格式: Affinity Biosciences Cat# DF6230, RRID:AB_2838196.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

58 kDa glucose regulated protein; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER 60; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER p57; ER protein 57; ER protein 60; ERp 57; ERp57; ERp60; ERp61; Glucose Regulated Protein 58 Kd; GRP 57; GRP 58; GRP57; HsT17083; p58; PDIA 3; PDIA3; PDIA3_HUMAN; Phospholipase C alpha; PI PLC; Protein disulfide isomerase A3; Protein disulfide isomerase family A member 3; Protein disulfide-isomerase A3;

抗原和靶标

免疫原:

Uniprot:

P30101(PDIA3_HUMAN) >>Visit HPA database.

基因/基因ID:

PDIA3(2923)

表达:

P30101 PDIA3_HUMAN:

Detected in the flagellum and head region of spermatozoa (at protein level).

描述:

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 57 (ERp57), constitute the PDI family (2). ERp57 interacts with calnexin and calreticulin (3) and is suggested to play a role in the isomerization of disulfide bonds on certain glycoproteins (3).

序列:

P30101 PDIA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRLRRLALFPGVALLLAAARLAAASDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Bovine

Sheep

Chicken

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P30101 作为底物

P30101

Site	PTM Type	Enzyme	Source
S37	Phosphorylation		Uniprot
K61	Methylation		Uniprot
Y67	Phosphorylation		Uniprot
R73	Methylation		Uniprot
K75	Ubiquitination		Uniprot
K82	Ubiquitination		Uniprot
C85	S-Nitrosylation		Uniprot
T86	Phosphorylation		Uniprot
C92	S-Nitrosylation		Uniprot
K94	Acetylation		Uniprot
K94	Ubiquitination		Uniprot
S98	Phosphorylation		Uniprot
Y100	Phosphorylation		Uniprot
T102	O-Glycosylation		Uniprot
T102	Phosphorylation		Uniprot
K104	Acetylation		Uniprot
K104	Ubiquitination		Uniprot
Y115	Phosphorylation		Uniprot
R119	Methylation		Uniprot
S126	Phosphorylation		Uniprot
S136	Phosphorylation		Uniprot
R140	Methylation		Uniprot
T141	Phosphorylation		Uniprot
S163	Phosphorylation		Uniprot
S165	Phosphorylation		Uniprot
K173	Ubiquitination		Uniprot
S176	Phosphorylation		Uniprot
R179	Methylation		Uniprot
Y196	Phosphorylation		Uniprot
K214	Ubiquitination		Uniprot
T223	Phosphorylation		Uniprot
K226	Acetylation		Uniprot
K226	Ubiquitination		Uniprot
C244	S-Nitrosylation		Uniprot
Y269	Phosphorylation		Uniprot
K289	Ubiquitination		Uniprot
K296	Ubiquitination		Uniprot
S303	Phosphorylation		Uniprot
K305	Ubiquitination		Uniprot
S312	Phosphorylation		Uniprot
S318	Phosphorylation		Uniprot
T319	Phosphorylation		Uniprot
R329	Methylation		Uniprot
K335	Ubiquitination		Uniprot
S343	Phosphorylation		Uniprot
Y356	Phosphorylation		Uniprot
K362	Acetylation		Uniprot
K362	Ubiquitination		Uniprot
K366	Ubiquitination		Uniprot
S367	Phosphorylation		Uniprot
S373	Phosphorylation		Uniprot
Y402	Phosphorylation		Uniprot
S424	Phosphorylation		Uniprot
K425	Ubiquitination		Uniprot
K433	Ubiquitination		Uniprot
S443	Phosphorylation		Uniprot
Y445	Phosphorylation		Uniprot
R448	Methylation		Uniprot
T452	O-Glycosylation		Uniprot
T452	Phosphorylation		Uniprot
Y454	Phosphorylation		Uniprot
S456	O-Glycosylation		Uniprot
S456	Phosphorylation	P06493 (CDK1)	Uniprot
S474	Phosphorylation		Uniprot
S478	Phosphorylation		Uniprot
Y479	Phosphorylation		Uniprot
T485	O-Glycosylation		Uniprot

研究背景

P30101_PDIA3_HUMAN

细胞定位:

Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).

组织特异性:

Detected in the flagellum and head region of spermatozoa (at protein level).

亚基结构:

Subunit of the TAP complex, also known as the peptide loading complex (PLC). Can form disulfide-linked heterodimers with TAPBP. Interacts with ERP27 and CANX. Interacts with SERPINA2 and with the S and Z variants of SERPINA1. Interacts with ATP2A2 (By similarity).

蛋白家族:

Belongs to the protein disulfide isomerase family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)

文献引用

1). Novel functional mutation of the PDIA3 gene affects milk composition traits in Chinese Holstein cattle. JOURNAL OF DAIRY SCIENCE, 2022 (PubMed: 35379459) [IF=3.5]

Application: IF/ICC Species: cow Sample:

Figure 4. Expression and subcellular localization of PDIA3. (A) mRNA expression profile of PDIA3 in different tissues of dairy cows by quantitative real-time PCR method; (B) subcellular localization of PDIA3 in bovine mammary alveolar cells observed by immunofluorescence assay. PDIA3 (green); endoplasmic reticulum (ER) signals (red); nucleus stained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI, blue). Scale bar represents 10 μm.

Application: WB Species: cow Sample:

Figure 5. Detection of the efficiency of PDIA3 overexpression and knockdown in bovine mammary alveolar cells. (A) Map of PDIA3 overexpression vector (left), and agarose gel electrophoresis of pCDNA3.1- PDIA3 vector digested with restriction endonucleases (right). M = DNA marker. Lane 1 (L1) shows 2 bands of 2,248 bp and 4,671 bp with Mlu I and Xho I digestion; lane 2 (L2) shows the pCDNA3.1- PDIA3 plasmid. (B) Cells were transfected for 48 h with pCDNA3.1- PDIA3 overexpression vector (OE), pCDNA3.1 empty vector (EV), PDIA3 siRNA (KD), or negative control siRNA (NC), and the relative mRNA levels of PDIA3 were analyzed by quantitative real-time PCR. (C) Protein levels of PDIA3 were determined by western blot analysis, and quantitative results show fold increases in PDIA3/GAPDH ratios. **P < 0.01, compared with control group; ns = not significant. Error bars represent SEM. siRNA = small interfering RNA.

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PDIA3 Antibody - #DF6230

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