产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6241, RRID:AB_2838207.
展开/折叠
Antigen p25; CBX5; CBX5_HUMAN; CG8409; Chromobox 5; Chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Epididymis luminal protein 25; HEL25; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha; Su(var)205;
抗原和靶标
- P45973 CBX5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKKTKRTADSSSSEDEEEYVVEKVLDRRVVKGQVEYLLKWKGFSEEHNTWEPEKNLDCPELISEFMKKYKKMKEGENNKPREKSESNKRKSNFSNSADDIKSKKKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLMKWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWHAYPEDAENKEKETAKS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P45973 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T8 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S12 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S13 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S14 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y20 | Phosphorylation | Uniprot | |
| K32 | Methylation | Uniprot | |
| K32 | Sumoylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| Y37 | Phosphorylation | Uniprot | |
| K40 | Acetylation | Uniprot | |
| K40 | Methylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K55 | Acetylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K91 | Acetylation | Uniprot | |
| K91 | Methylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| S92 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S95 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| K102 | Sumoylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| R115 | Methylation | Uniprot | |
| R119 | Methylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| K143 | Acetylation | Uniprot | |
| K143 | Methylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K154 | Methylation | Uniprot | |
| K154 | Sumoylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| S191 | Phosphorylation | Uniprot |
研究背景
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated.
Nucleus. Chromosome. Chromosome>Centromere.
Note: Colocalizes with HNRNPU in the nucleus (PubMed:19617346). Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
Interacts with KMT5B and KMT5C (By similarity). Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with CHAMP1. Interacts with PRR14 (via N-terminus). Interacts with RRP1B. Interacts with HNRNPU (via C-terminus); this interaction is, at least in part, RNA-dependent. Interacts with LRIF1 (via PxVxL motif).
(Microbial infection) Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope.
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