GPX1 Antibody - #DF6249

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产品: GPX1 抗体
货号: DF6249
描述: Rabbit polyclonal antibody to GPX1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog, Chicken
分子量: 22kDa; 22kD(Calculated).
蛋白号: P07203
RRID: AB_2838215

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(82%), Horse(100%), Rabbit(90%), Dog(90%), Chicken(80%)
克隆:
Polyclonal
特异性:
GPX1 Antibody detects endogenous levels of total GPX1.
RRID:
AB_2838215
引用格式: Affinity Biosciences Cat# DF6249, RRID:AB_2838215.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AL033363; Cellular glutathione peroxidase; Glutathione peroxidase 1; Glutathione peroxidase; GPx 1; GPx-1; GPX1; GPX1_HUMAN; GPXD; GSHPx-1; GSHPX1; MGC14399; MGC88245;

抗原和靶标

免疫原:

A synthesized peptide derived from human GPX1, corresponding to a region within the internal amino acids.

Uniprot:

P07203(GPX1_HUMAN) >>Visit HPA database.

基因/基因ID:
GPX1(2876)
描述:
Glutathione peroxidase 1 (GPX1) is a cytosolic selenoprotein which reduces hydrogen peroxide to water (1). GPX1 is the most abundant and ubiquitous among the five GPX isoforms identified so far (2). It is an important component in the anti-oxidative defense in cells and is associated with a variety of disease conditions, such as colon cancer (3), coronary artery disease (4) and insulin resistance (1).
序列:
MCAARLAAAAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLUGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Pig
91
Dog
90
Rabbit
90
Bovine
82
Chicken
80
Xenopus
64
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07203 作为底物

Site PTM Type Enzyme
S34 Phosphorylation
K88 Acetylation
K97 Ubiquitination
Y98 Phosphorylation P42684 (ABL2) , P00519 (ABL1)
T145 Phosphorylation
K148 Acetylation
S153 Phosphorylation
K166 Ubiquitination
S180 Phosphorylation
S201 Phosphorylation
C202 S-Nitrosylation

研究背景

功能:

Protects the hemoglobin in erythrocytes from oxidative breakdown.

翻译修饰:

During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homotetramer. Interacts with MIEN1.

蛋白家族:

Belongs to the glutathione peroxidase family.

研究领域

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

文献引用

1). Arsenic retention in erythrocytes and excessive erythrophagocytosis is related to low selenium status by impaired redox homeostasis. Redox Biology, 2022 (PubMed: 35500533) [IF=11.4]
2). An optimal combination of five main monomer components in Wuzi Yanzong Pill that prevents neural tube defects and reduces apoptosis and oxidative stress. Journal of Ethnopharmacology, 2023 [IF=5.4]
3). Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. FOOD AND CHEMICAL TOXICOLOGY, 2017 (PubMed: 28137608) [IF=4.3]

Application: WB    Species: rat    Sample: PC12 cells

4). Antioxidative effect of Lactobacillus plantarum Y44 on 2,2\'-azobis(2-methylpropionamidine) dihydrochloride (ABAP)-damaged Caco-2 cells. JOURNAL OF DAIRY SCIENCE, 2019 (PubMed: 31178173) [IF=3.5]
5). N-acetylcysteine maintains penile length and erectile function in bilateral cavernous nerve crush rat model by reducing penile fibrosis. Asian Journal of Andrology, 2021 (PubMed: 32394901) [IF=2.9]

Application: WB    Species: Rat    Sample:

Figure 4 Oxidative markers for corpus cavernosum. (a) Result of LOX expression of Western blot. (b) LOX enzyme activity. (c) Representative result of GPX1 expression. Protein expression in a and c was determined by densitometry relative to GAPDH. (d) Measurement of GSH. (e) Statistical analysis of MDA. Compared with the BCNC group, the NAC therapy group significantly decreased the expression and activity of LOX (P <0.05). GPX1 had an upward trend after NAC treatment, but there was no statistical difference (P = 0.0902). The over-expression of MDA was significantly reduced (P <0.05), while the expression of GSH was increased (P <0.05). *P <0.05. BCNC: bilateral cavernous nerve crush; GAPDH: glyceraldehyde phosphate dehydrogenase; GPX1: glutathione peroxidase 1; GSH: glutathione; LOX: lysine oxidase; MDA: malonaldehyde; NAC: N-acetylcysteine; RFUs: relative fluorescent units.
6). Short Photoperiod Reduces Oxidative Stress by Up-Regulating the Nrf2–Keap1 Signaling Pathway in Hamster Kidneys. Journal of Evolutionary Biochemistry and Physiology, 2022 [IF=0.6]

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