CYR61/CCN1 Antibody - #DF6250

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产品: CYR61/CCN1 抗体
货号: DF6250
描述: Rabbit polyclonal antibody to CYR61/CCN1
应用: WB IHC IF/ICC
文献应用: WB, IHC
反应: Human, Mouse, Rat
文献反应: Human
预测: Rabbit
分子量: 42kDa; 42kD(Calculated).
蛋白号: O00622
RRID: AB_2838216

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Rabbit(88%)
克隆:
Polyclonal
特异性:
CYR61/CCN1 Antibody detects endogenous levels of total CYR61/CCN1.
RRID:
AB_2838216
引用格式: Affinity Biosciences Cat# DF6250, RRID:AB_2838216.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

3CH61; CCN 1; CCN family member 1; CYR 61; Cyr61; CYR61 protein; CYR61_HUMAN; Cysteine rich angiogenic inducer 61; Cysteine rich heparin binding protein 61; Cysteine-rich angiogenic inducer 61; GIG 1; GIG1; IBP-10; IGF-binding protein 10; Igfbp 10; IGFBP-10; Igfbp10; Insulin like growth factor binding protein 10; Insulin-like growth factor-binding protein 10; Protein CYR61; Protein GIG1;

抗原和靶标

免疫原:
Uniprot:

O00622(CCN1_HUMAN) >>Visit HPA database.

基因/基因ID:
CYR61(3491)
描述:
Cyr61 is a secreted heparin binding protein, encoded by a growth factor-inducible immediate-early gene, that associates with the extracellular matrix and connective tissue. Cyr61 is a member of a distinct family of angiogenic and vasculogenic regulators designated CCN proteins, which includes connective tissue growth factor (CTGF) and the mouse Cyr61 homolog, Fisp12. As an angiogenic inducer, Cyr61 binds to the cell surface receptor Integrin aV/b3, where it then stimulates cell adhesion and migration and promotes DNA synthesis of human vascular endothelial cells. Expression of Cyr61 is elevated during vessel growth, wound healing and chondrocyte differentiation. Cyr61 is also detected in a wide variety of tumors as it induces tumor growth and functions as a marker of tumor progression.
序列:
MSSRIARALALVVTLLHLTRLALSTCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVTGQCCEEWVCDEDSIKDPMEDQDGLLGKELGFDASEVELTRNNELIAVGKGSSLKRLPVFGMEPRILYNPLQGQKCIVQTTSWSQCSKTCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCLSVKKYRPKYCGSCVDGRCCTPQLTRTVKMRFRCEDGETFSKNVMMIQSCKCNYNCPHANEAAFPFYRLFNDIHKFRD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
88
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00622 作为底物

Site PTM Type Enzyme
T14 Phosphorylation
S84 Phosphorylation
T85 Phosphorylation
K88 Ubiquitination
S167 Phosphorylation
S188 Phosphorylation
K203 Ubiquitination
Y221 Phosphorylation
Y277 Phosphorylation
S278 Phosphorylation
K281 Ubiquitination

研究背景

功能:

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interaction with integrins is heparin- and cell-type-dependent and promotes cell adhesion. In skin fibroblasts it binds ITGA6/ITGB1, in endothelial cells, binds ITGAV/ITGB3 and in platelets, ITGA2B/ITGB3. Binds, in vitro, ITGAV/ITGB5.

蛋白家族:

Belongs to the CCN family.

文献引用

1). Downregulation of miR-142-5p promotes tumor metastasis through directly regulating CYR61 expression in gastric cancer. Gastric Cancer, 2019 (PubMed: 30178386) [IF=6.0]

Application: IHC    Species: human    Sample: gastric and GC tissues

Fig. 5| High expression of CYR61 was associated with prognosis in GC. a The expression of CYR61 in normal gastric tissues (upper) and GC tissues (lower) (left: magnification ×200; right: magnification ×400).

Application: WB    Species: human    Sample: BGC823 and SGC7901 cells， AGS cells

Fig. 3 |CYR61 is a novel target gene of miR-142-5p. c Left and middle, qRT-PCR and western blot showed that the expression of CYR61 was significantly decreased in miR-142-5p-stably transfected BGC823 and SGC7901 cells as compared with control cells. Right, qRT-PCR and western blot showed that the expression of CYR61 was significantly increased in miR142-5p-inhibitor-stably transfected AGS cells as compared with control cells. *P < 0.05, **P < 0.01, ***P < 0.001.
2). Upregulation of Angiomotin-like 2 ameliorates experimental pulmonary arterial hypertension by inactivating YAP1 signaling. Journal of cardiovascular pharmacology, 2022 (PubMed: 39027975) [IF=2.6]

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