| 产品: | Emerin 抗体 |
| 货号: | DF6266 |
| 描述: | Rabbit polyclonal antibody to Emerin |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog |
| 分子量: | 29kDa; 29kD(Calculated). |
| 蛋白号: | P50402 |
| RRID: | AB_2838232 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6266, RRID:AB_2838232.
展开/折叠
EDMD; Emd; EMD_HUMAN; Emerin; Emery Dreifuss muscular dystrophy; STA;
抗原和靶标
- P50402 EMD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRLSPPSSSAASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGNPF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P50402 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y4 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| Y19 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) | Uniprot |
| S29 | Phosphorylation | Uniprot | |
| Y34 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| Y41 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) | Uniprot |
| T43 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S52 | Phosphorylation | Uniprot | |
| S53 | O-Glycosylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S54 | O-Glycosylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| Y59 | Phosphorylation | P12931 (SRC) , P00519 (ABL1) | Uniprot |
| S60 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| Y74 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) | Uniprot |
| K78 | Ubiquitination | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| Y85 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) | Uniprot |
| S87 | O-Glycosylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| Y90 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) | Uniprot |
| Y94 | Phosphorylation | P12931 (SRC) , P00519 (ABL1) | Uniprot |
| Y95 | Phosphorylation | P12931 (SRC) , P00519 (ABL1) | Uniprot |
| S98 | Phosphorylation | Uniprot | |
| Y99 | Phosphorylation | P12931 (SRC) , P00519 (ABL1) | Uniprot |
| T101 | Phosphorylation | Uniprot | |
| R103 | Methylation | Uniprot | |
| T104 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | P12931 (SRC) , P00519 (ABL1) | Uniprot |
| S110 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| T122 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| Y155 | Phosphorylation | P00519 (ABL1) | Uniprot |
| S159 | Phosphorylation | Uniprot | |
| Y161 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) | Uniprot |
| S163 | Phosphorylation | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| Y167 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) | Uniprot |
| S171 | O-Glycosylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S173 | O-Glycosylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| Y181 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y182 | Phosphorylation | P12931 (SRC) | Uniprot |
| S189 | Phosphorylation | Uniprot |
研究背景
Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.
Nucleus inner membrane>Single-pass membrane protein>Nucleoplasmic side. Nucleus outer membrane.
Note: Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus.
Skeletal muscle, heart, colon, testis, ovary and pancreas.
Interacts with lamins A and C, BANF1, GMCL, BCLAF1 and YTHDC1/YT521. Interacts with TMEM43; the interaction retains emerin in the nuclear inner membrane. Interacts with SUN1 and SUN2 (By similarity). Interacts with ACTB, SPTAN1, F-actin, CTNNB1 and beta-tubulin. Interacts with TMEM201.
研究领域
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
限制条款
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