| 产品: | FBL 抗体 |
| 货号: | DF6270 |
| 描述: | Rabbit polyclonal antibody to FBL |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 34kDa; 34kD(Calculated). |
| 蛋白号: | P22087 |
| RRID: | AB_2838236 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6270, RRID:AB_2838236.
展开/折叠
34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; Fbl; FBRL_HUMAN; FIB; FIB1; FLRN; Histone-glutamine methyltransferase; Nop1p; RNA U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2' O methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin;
抗原和靶标
- P22087 FBRL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P22087 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| R8 | Methylation | Uniprot | |
| R15 | Methylation | Uniprot | |
| R21 | Methylation | Uniprot | |
| R24 | Methylation | Uniprot | |
| R27 | Methylation | Uniprot | |
| R34 | Methylation | Uniprot | |
| R36 | Methylation | Uniprot | |
| R45 | Methylation | Uniprot | |
| R59 | Methylation | Uniprot | |
| R70 | Methylation | Uniprot | |
| R72 | Methylation | Uniprot | |
| R78 | Methylation | Uniprot | |
| C99 | S-Nitrosylation | Uniprot | |
| K102 | Acetylation | Uniprot | |
| K102 | Sumoylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K109 | Sumoylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| K121 | Acetylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K143 | Sumoylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K158 | Sumoylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K205 | Acetylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| Y302 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot |
研究背景
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.
Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36.
By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation. Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me).
Nucleus>Nucleolus.
Note: Fibrillar region of the nucleolus.
Interacts with NOLC1 (By similarity). Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain SNU13, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5 and C1QBP. Interacts with NOL11. Interacts with PIH1D1. Interacts with RRP1B.
Belongs to the methyltransferase superfamily. Fibrillarin family.
研究领域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.