MCM7 Antibody - #DF6272

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产品: MCM7 抗体
货号: DF6272
描述: Rabbit polyclonal antibody to MCM7
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Rabbit, Dog, Xenopus
分子量: 81kDa; 81kD(Calculated).
蛋白号: P33993
RRID: AB_2838238

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(100%), Bovine(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
MCM7 Antibody detects endogenous levels of total MCM7.
RRID:
AB_2838238
引用格式: Affinity Biosciences Cat# DF6272, RRID:AB_2838238.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDABP0042; CDC 47; CDC47; CDC47 homolog; Cdc47, S. cerevisiae, homolog of; DNA replication licensing factor MCM7; Homolog of S. cerevisiae Cdc47; MCM 2; MCM 7; MCM2; MCM2, formerly; Mcm7; MCM7 minichromosome maintenance deficient 7; MCM7_HUMAN; Minichromosome Maintainence 7; Minichromosome maintainence, S. cerevisiae, homolog of; Minichromosome maintenance complex component 7; Minichromosome maintenance deficient 7; Minichromosome maintenance protein 7; P1.1 MCM3; P1.1-MCM3; P1CDC47; P85MCM; PNAS 146; PNAS146; PPP1R104; Protein phosphatase 1, regulatory subunit 104;

抗原和靶标

免疫原:
Uniprot:

P33993(MCM7_HUMAN) >>Visit HPA database.

基因/基因ID:
MCM7(4176)
描述:
The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).
序列:
MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSNKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIAEEDFYEKLAASIAPEIYGHEDVKKALLLLLVGGVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLRDSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNARCSILAAANPAYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPSQFEPLDMKLMRRYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATYTSARTLLAILRLSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
75
Pig
0
Horse
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P33993 作为底物

Site PTM Type Enzyme
A2 Acetylation
K4 Sumoylation
K4 Ubiquitination
Y6 Phosphorylation
K10 Sumoylation
K10 Ubiquitination
K15 Sumoylation
K15 Ubiquitination
Y21 Phosphorylation
K28 Acetylation
K28 Ubiquitination
K29 Acetylation
K29 Ubiquitination
K32 Sumoylation
K32 Ubiquitination
K75 Ubiquitination
K89 Ubiquitination
K96 Ubiquitination
Y102 Phosphorylation
S113 Phosphorylation
S121 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
K145 Ubiquitination
S156 Phosphorylation
K159 Sumoylation
K159 Ubiquitination
T168 Phosphorylation
K174 Sumoylation
K174 Ubiquitination
R219 Methylation
R225 Methylation
K231 Sumoylation
K231 Ubiquitination
K236 Sumoylation
K236 Ubiquitination
Y297 Phosphorylation
K305 Ubiquitination
K308 Sumoylation
K308 Ubiquitination
S309 Phosphorylation
S314 Phosphorylation
T320 Phosphorylation
R321 Methylation
R325 Methylation
Y333 Phosphorylation
K335 Ubiquitination
K351 Ubiquitination
K352 Ubiquitination
S365 Phosphorylation P24941 (CDK2)
K387 Ubiquitination
S388 Phosphorylation
S392 Phosphorylation
R396 Methylation
R407 Methylation
K449 Methylation
K471 Ubiquitination
C482 S-Nitrosylation
Y492 Phosphorylation
S500 Phosphorylation
R524 Methylation
R527 Methylation
R532 Methylation
T538 Phosphorylation
Y539 Phosphorylation
S544 Phosphorylation
S549 Phosphorylation
K557 Ubiquitination
K569 Ubiquitination
K596 Ubiquitination
T599 Phosphorylation
Y600 Phosphorylation P07948 (LYN)
T605 Phosphorylation
K627 Ubiquitination
K641 Ubiquitination
K648 Ubiquitination
S670 Phosphorylation
R673 Methylation
S674 Phosphorylation
S678 Phosphorylation

研究背景

功能:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

翻译修饰:

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. Interacts with ANKRD17. Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR.

蛋白家族:

Belongs to the MCM family.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

文献引用

1). ALKBH5-mediated m 6 A modification of CDC6 mRNA impairs gastric cancer tumorigenesis and has prognostic significance. Research Square, 2023

Application: WB    Species: Human    Sample: MKN-28 cells

Figure 3. RNA-seq analysis of the enrichment of ALKBH5 in GC proliferation and cycle progression. (A) RNA-seq and volcano plotting of the DEGs between ALKBH5 and control groups and their distribution on human chromosomes. (B) KEGG analysis of the enrichment pathways by the upregulated or downregulated genes caused by ALKBH5-OE in GC cells. (C) GO analysis of the enriched biological function of the upregulated or downregulated genes caused by ALKBH5-OE in GC cells. (D) Venn analysis of the intersection of Cell cycle and DNA replication-related genes (n = 147) and DEGs from the KEGG dataset (n = 159). (E) Bubble chart displaying the expression levels of cell proliferation and cycle-related 7 target genes regulated by ALKIBH5 in MKN-28 cells. (F) Western blotting analysis of the effects of ALKBH5 overexpression on the protein levels of 7 target genes in MKN-28 cells. (G) TCGA and (H) GEPIA databases uncovering the expression levels of 7 target genes regulated by ALKBH5 in GC tissue samples.

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