产品: FTH1 抗体
货号: DF6278
描述: Rabbit polyclonal antibody to FTH1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Rabbit, Dog, Chicken, Xenopus
分子量: 21kDa; 21kD(Calculated).
蛋白号: P02794
RRID: AB_2838244

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Rabbit(90%), Dog(90%), Chicken(80%), Xenopus(88%)
克隆:
Polyclonal
特异性:
Ferritin Heavy Chain Antibody detects endogenous levels of total Ferritin Heavy Chain.
RRID:
AB_2838244
引用格式: Affinity Biosciences Cat# DF6278, RRID:AB_2838244.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Apoferritin; Cell proliferation inducing gene 15 protein; Cell proliferation-inducing gene 15 protein; F HC; Ferritin H subunit; Ferritin heavy chain; Ferritin heavy polypeptide 1; FHC; FRIH; FRIH_HUMAN; FTH 1; FTH; FTH1; FTH1 protein; FTHL 6; FTHL6; Iron overload autosomal dominant; MGC104426; N-terminally processed; OK/SW-cl.84; PIG 15; PIG15; Placenta immunoregulatory factor; PLIF; Proliferation inducing gene 15 protein; Proliferation inducing protein 15;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P02794 FRIH_HUMAN:

Expressed in the liver.

描述:
Ferritin (FTH) is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and bioavailable form (1). The assembled ferritin molecule, often referred to as a nanocage, can store up to 4,500 atoms of iron (2,3). It forms a holoenzyme of ~450 kDa, consisting of 24 subunits made up of two types of polypeptide chains: ferritin heavy chain and ferritin light chain, each having unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III) (4). In addition to iron buffering, heavy chain ferritin also enhances thymidine biosynthesis (5). Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia. The level of serum ferritin is markedly elevated in inflammation, malignancy, and iron overload disorders (6). Research studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases (7).
序列:
MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
90
Dog
90
Xenopus
88
Chicken
80
Horse
78
Pig
70
Bovine
70
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P02794 作为底物

Site PTM Type Enzyme
M1 Acetylation
T2 Acetylation
T6 Phosphorylation
S7 Phosphorylation
Y13 Phosphorylation
K54 Ubiquitination
K69 Ubiquitination
K72 Sumoylation
K72 Ubiquitination
K88 Ubiquitination
S96 Phosphorylation
K109 Ubiquitination
S114 Phosphorylation
K120 Ubiquitination
K125 Ubiquitination
K144 Ubiquitination
K147 Ubiquitination
K158 Ubiquitination
S164 Phosphorylation
Y169 Phosphorylation
T175 Phosphorylation
S179 Phosphorylation
S183 Phosphorylation

研究背景

功能:

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

组织特异性:

Expressed in the liver.

亚基结构:

Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.

蛋白家族:

Belongs to the ferritin family.

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

文献引用

1). VDR Activation Attenuates Renal Tubular Epithelial Cell Ferroptosis by Regulating Nrf2/HO-1 Signaling Pathway in Diabetic Nephropathy. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38145959) [IF=15.1]

Application: WB    Species: human    Sample: HK‐2 cells

Figure 2 VDR activation suppressed ferroptosis in HG‐cultured HK‐2 cells. The mitochondrial morphology of HK‐2 cells was visualized by TEM. The arrow indicates microscopic changes in mitochondria. Absolute counting of the number of cristae per mitochondria. Scale bar = 2 or 0.5 µm (A). Quantitative analysis of iron (B), MDA (C), and GSH (D) levels in each cell group. The relative mRNA levels of SLC7A11, GPX4, TFRC,F TH1, and AIFM2 in different groups of HK‐2 cells were determined by qRT‐PCR. ACTB served as a loading control (E). Western blotting was applied to detect the protein expression levels of SLC7A11, GPX4, TFR‐1, FTH‐1, and FSP1, followed by densitometric analysis of the blots. β‐actin served as a loading control (F). Immunofluorescence staining and fluorescence intensity analysis of SLC7A11, GPX4, and TFR‐1 in different groups of HK‐2 cells as indicated. Scale bar = 20 µm (G). Each bar represents the mean ± SD of the data derived from three independent experiments (n = 3). ** p < 0.01 versus NG group; # p < 0.05, ## p < 0.01 versus HG group; & p < 0.05, && p < 0.01 versus HG group.

2). Activation of integrated stress response and disordered iron homeostasis upon combined exposure to cadmium and PCB77. JOURNAL OF HAZARDOUS MATERIALS, 2020 (PubMed: 31837937) [IF=13.6]

Application: WB    Species: Human    Sample: Human erythroleukemia cell lines (HEL)

Fig. 5. Disordered iron homeostasis and inhibited mTORC1 activity upon exposure to CdCl2 and PCB77 at low dose. (A) The relative fluorescence intensity of CAeAM for measuring LIP to reflect intracellular iron availability (n = 3–4), and (B) Representative blots of FTH1 protein content to reflect iron storage. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. (C) Phosphorylated S6 and total S6 content to re- flect mTORC1 activity as measured by Western blot. Ratio of FTH1 to eIF2αP and ratio of pS6 to S6 in the control group were defined as 1. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. a- significantly different from the control group. Data were presented in mean ± SE. P < 0.05 was considered statistically significant.

3). Silibinin attenuates ferroptosis in acute kidney injury by targeting FTH1. Redox biology, 2024 (PubMed: 39326069) [IF=11.4]

Application: IF/ICC    Species: Mouse    Sample: kidney

Fig. 5. FTH1 plays a critical role in silibinin-mediated inhibition of ferroptosis, and silibinin inhibits ferroptosis by disrupting the NCOA4-FTH1 interaction. (A) The schematic diagram of identifying silibinin target proteins. (B) Venn diagram of silibinin target proteins and biotin target proteins. (C) Representative images of FTH1 in the proteome microarray. (D) Z-score and IMean ratio of FTH1-silibinin binding. (E) Molecular docking of FTH1 and silibinin. (F) SPRi fitting curves for silibinin to FTH1. (G–H) CETSA-Western blot analysis showed the protection of FTH1 by silibinin at different temperature gradients (n = 3). (I–J) DARTS-Western blot analysis showed the resistance of FTH1 to pronase digestion under the treatment of silibinin (n = 3). (K–M) Western bolt analysis and quantitative results of NCOA4 and FTH1 (n = 4). (N) Representative immunofluorescence images of FTH1 and NCOA4 co-staining. (O) Co-IP assay of NCOA4 and FTH1 interaction in the kidney of IRI mice. (P) Quantitative results of co-IP assay showing the endogenous interaction between NCOA4 and FTH1 (n = 3). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Application: WB    Species: Mouse    Sample: kidney

Fig. 5. FTH1 plays a critical role in silibinin-mediated inhibition of ferroptosis, and silibinin inhibits ferroptosis by disrupting the NCOA4-FTH1 interaction. (A) The schematic diagram of identifying silibinin target proteins. (B) Venn diagram of silibinin target proteins and biotin target proteins. (C) Representative images of FTH1 in the proteome microarray. (D) Z-score and IMean ratio of FTH1-silibinin binding. (E) Molecular docking of FTH1 and silibinin. (F) SPRi fitting curves for silibinin to FTH1. (G–H) CETSA-Western blot analysis showed the protection of FTH1 by silibinin at different temperature gradients (n = 3). (I–J) DARTS-Western blot analysis showed the resistance of FTH1 to pronase digestion under the treatment of silibinin (n = 3). (K–M) Western bolt analysis and quantitative results of NCOA4 and FTH1 (n = 4). (N) Representative immunofluorescence images of FTH1 and NCOA4 co-staining. (O) Co-IP assay of NCOA4 and FTH1 interaction in the kidney of IRI mice. (P) Quantitative results of co-IP assay showing the endogenous interaction between NCOA4 and FTH1 (n = 3). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

4). Identification of ZIP8-induced ferroptosis as a major type of cell death in monocytes under sepsis conditions. Redox biology, 2024 (PubMed: 38103342) [IF=11.4]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Fig. 5 ZIP8 regulates ferroptosis of monocytes under sepsis conditions in vitro. a. Representative immunoblots of ZIP8-G, ZIP8-N, GPX4, FTH1 and xCT in RAW264.7 cells treated with different concentrations of LPS and durations. b-f. Immunoblot analyses of ZIP8-G (b, n = 4/group), ZIP8-N (c, n = 4/group), GPX4 (d, n = 4/group), FTH1 (e, n = 4/group), and xCT (f, n = 4/group) exhibited a sequential pattern of expression changes between ZIP8 (peaked at 6 h post-treatment) and ferroptosis related GPX4 and FTH1 (hit the lowest at 12 h post-treatment). g. Representative immunoblots of ZIP8-G, ZIP8-N, GPX4, FTH1 and xCT in RAW264.7 cells with/without decreased Slc39a8 expression and/or LPS treatment. h-l. Immunoblot analyses of ZIP8-G (h, n = 5/group), ZIP8-N (i, n = 5/group), GPX4 (j, n = 5/group), FTH1 (k, n = 5/group), and xCT (l, n = 5/group) exhibited that suppressing ZIP8 attenuated the decrease of GPX4, FTH1 and xCT in response to LPS. m-o. Quantification analysis showed that decreasing ZIP8 alleviated the LPS-induced upregulations of MDA (m, n = 6/group) and ferrous ions (o, n = 6/group) and reversed the decreased GSH level (n, n = 6/group). One-way ANOVA was performed in b-f. Two-way ANOVA was performed in h-o. Compared to PBS treated si-NC group:

5). ZnO NPs induce miR-342-5p mediated ferroptosis of spermatocytes through the NF-κB pathway in mice. JOURNAL OF NANOBIOTECHNOLOGY, 2024 [IF=10.8]

Application: WB    Species: Mouse    Sample: GC-2 cells

Fig. 6 ZnO NPs induce the ferroptosis of GC-2 cells. (A) Intracellular chelatable iron in GC-2 cells treated with or without ZnO NPs stained with PGSK (green). Statistical analysis of MFI of PGSK was shown. (B) Representative FACS data for lipid peroxidation level in GC-2 cells following ZnO NPs treatment using C11 BODIPY. Statistical analysis of MFI of the ratio of green/red was shown. (C) qRT-PCR analysis of ferroptosis-related gene expression in GC-2 cells after ZnO NPs treatment. (D) Western blot of ferroptosis-related protein levels in GC-2 cells treated with ZnO NPs. Statistical analysis of mean grey values ratios of the corresponding proteins/β-actin was shown, the same as below. (E) The cell viability of GC-2 cells following ZnO NPs treatment with or without Fer-1 (3.5 µM). (F) Intracellular chelatable iron in GC-2 cells following ZnO NPs treatment with or without Fer-1 stained with PGSK. Statistical analysis of MFI of PGSK was shown. (G) Representative FACS data of lipid peroxidation level in GC-2 cells following ZnO NPs treatment with or without Fer-1 stained with C11 BODIPY. Statistical analysis of MFI of the ratio of green/red was shown. (H and I) The levels of GSH and MDA in GC-2 cells following ZnO NPs treatment with or without Fer-1. (J) qRT-PCR analysis of ferroptosis-related gene expression in GC-2 cells following ZnO NPs treatment with or without Fer-1. (K) Western blot of ferroptosis-related protein levels in GC-2 cells following ZnO NPs treatment with or without Fer-1

6). Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1. BASIC RESEARCH IN CARDIOLOGY, 2021 (PubMed: 33484341) [IF=9.5]

Application: IHC    Species: mouse    Sample: heart

Fig. 5| Overexpression of BCAT1 and BCAT2 ameliorated AMIinduced myocardial injury. All mice were killed 24 h after the left anterior descending CAL. The heart-specifc BCAT1 or BCAT2 over-expression mice were constructed by direct injecting Adenoassociated virus (AAV) vectors carrying BCAT1 or BCAT2 in the LV free wall in mice using a syringe with a 30-gauge needle.h Representative immunohistochemical analysis of the expression of FHC, FLC, GPX4 and Caspase-3 in heart tissue of sham and model mice with cardiac overexpression of BCAT1 and BCAT2 (n = 3 mice) (400 ×  magnifcation, scale bar, 50 µm). #p < 0.05, ##p < 0.01 versus sham group, *p < 0.05, **p < 0.01 versus model group

7). Suppression of the SLC7A11/glutathione axis causes ferroptosis and apoptosis and alters the mitogen-activated protein kinase pathway in nasopharyngeal carcinoma. International journal of biological macromolecules, 2024 (PubMed: 37951442) [IF=8.2]

8). MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38569295) [IF=7.9]

9). Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib. Phytomedicine, 2023 (PubMed: 36657316) [IF=7.9]

10). Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38412575) [IF=7.9]

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