| 产品: | MAD1L1 抗体 |
| 货号: | DF6287 |
| 描述: | Rabbit polyclonal antibody to MAD1L1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Dog |
| 分子量: | 83kDa; 83kD(Calculated). |
| 蛋白号: | Q9Y6D9 |
| RRID: | AB_2838253 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6287, RRID:AB_2838253.
展开/折叠
hMAD1; HsMAD1; MAD1; MAD1 mitotic arrest deficient like 1 (yeast); MAD1-like protein 1; MAD1L1; MD1L1_HUMAN; Mitotic arrest deficient 1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; Mitotic spindle assembly checkpoint protein MAD1; PIG9; Tax binding protein 181; Tax-binding protein 181; TP53I9; Tumor protein p53 inducible protein 9; TXBP181;
抗原和靶标
- Q9Y6D9 MD1L1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDLGENTMVLSTLRSLNNFISQRVEGGSGLDISTSAPGSLQMQYQQSMQLEERAEQIRSKSHLIQVEREKMQMELSHKRARVELERAASTSARNYEREVDRNQELLTRIRQLQEREAGAEEKMQEQLERNRQCQQNLDAASKRLREKEDSLAQAGETINALKGRISELQWSVMDQEMRVKRLESEKQELQEQLDLQHKKCQEANQKIQELQASQEARADHEQQIKDLEQKLSLQEQDAAIVKNMKSELVRLPRLERELKQLREESAHLREMRETNGLLQEELEGLQRKLGRQEKMQETLVGLELENERLLAKLQSWERLDQTMGLSIRTPEDLSRFVVELQQRELALKDKNSAVTSSARGLEKARQQLQEELRQVSGQLLEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDSELTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKMLKSQSSSAEQSFLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSIPAFLSSLTLELFSRQTVA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6D9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S29 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K61 | Acetylation | Uniprot | |
| K61 | Sumoylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K143 | Acetylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K231 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| T423 | Phosphorylation | Uniprot | |
| Y427 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K478 | Acetylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| T500 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| Y535 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| K614 | Ubiquitination | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| T624 | Phosphorylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| Y634 | Phosphorylation | Uniprot | |
| T644 | Phosphorylation | Uniprot | |
| T680 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S699 | Phosphorylation | Uniprot | |
| T708 | Phosphorylation | Uniprot | |
| T716 | Phosphorylation | Uniprot |
研究背景
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
Nucleus. Chromosome>Centromere>Kinetochore. Nucleus envelope. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Detected at the nucleus envelope during interphase (PubMed:22351768). From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Detected at kinetochores during prometaphase (PubMed:22351768). Colocalizes with NEK2 at the kinetochore (PubMed:14978040). Colocalizes with IK at spindle poles during metaphase and anaphase (PubMed:22351768).
Expressed weakly at G0/G1 and highly at late S and G2/M phase.
Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex. Interacts with NEK2. Interacts with TPR; the interactions occurs in a microtubule-independent manner. Interacts with IK. Interacts with the viral Tax protein.
Belongs to the MAD1 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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