TPM1 Antibody - #DF6291

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产品: TPM1 抗体
货号: DF6291
描述: Rabbit polyclonal antibody to TPM1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 33kDa; 33kD(Calculated).
蛋白号: P09493
RRID: AB_2838257

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(83%)
克隆:
Polyclonal
特异性:
TPM1 Antibody detects endogenous levels of total TPM1.
RRID:
AB_2838257
引用格式: Affinity Biosciences Cat# DF6291, RRID:AB_2838257.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AA986836; AI854628; Alpha tropomyosin; alpha-TM; Alpha-tropomyosin; C15orf13; cardiomyopathy, hypertrophic 3; CMD1Y; CMH3; HTM alpha; HTM-alpha; OTTHUMP00000163688; sarcomeric tropomyosin kappa; TM2; Tmpa; TMSA; Tpm-1; TPM1; TPM1_HUMAN; tropomyosin 1 (alpha); tropomyosin 1 (alpha) isoform 1; tropomyosin 1 (alpha) isoform 2; tropomyosin 1 (alpha) isoform 3; tropomyosin 1 (alpha) isoform 4; tropomyosin 1 (alpha) isoform 5; tropomyosin 1 (alpha) isoform 6; tropomyosin 1 (alpha) isoform 7; Tropomyosin 1; Tropomyosin alpha 1 chain; Tropomyosin alpha-1 chain; Tropomyosin, skeletal muscle alpha; Tropomyosin-1;

抗原和靶标

免疫原:
Uniprot:

P09493(TPM1_HUMAN) >>Visit HPA database.

基因/基因ID:
TPM1(7168)
表达:
P09493 TPM1_HUMAN:

Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

描述:
Tropomyosin-1 (TPM1) belongs to the high molecular weight members of tropomyosin family (1,2). The protein exists in an alpha-helical coiled-coil conformation and binds multiple acting monomers in a tight manner to stabilize and regulate the actin filament (3). Tropomyosins fullfill functions in muscle and non-muscle cells. In muscle cells, tropomyosins associate with the troponin complex and play a central role in the calcium-dependent regulation of striated muscle contraction in vertebrates. In non-muscle cells, tropomyosins are implicated in the formation and stabilization of cytoskeletal actin filaments to ensure normal cellular processes (1,2). Mutations of tropomysin-1 have been reported as a cause of dilated cardiac myopathies (4). Tropomyosin-1 also functions as a tumor suppressor, and many malignant tumors demonstrate downregulation of tropomyosin-1 expression (5-8). Tropomyosin-1 is phosphorylated at Ser283 through the Erk/DAPK pathway, which promotes stress fiber formation in response to oxidative stress (9-10).
序列:
MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Pig
92
Chicken
92
Xenopus
83
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P09493 作为底物

Site PTM Type Enzyme
M1 Acetylation
K12 Acetylation
K12 Ubiquitination
K15 Acetylation
K15 Ubiquitination
K29 Acetylation
K29 Ubiquitination
K30 Ubiquitination
K37 Ubiquitination
S45 Phosphorylation
K48 Acetylation
K48 Ubiquitination
T53 Phosphorylation
K59 Ubiquitination
Y60 Phosphorylation
S61 Phosphorylation P42336 (PIK3CA)
K77 Ubiquitination
T79 Phosphorylation
S87 Phosphorylation
T108 Phosphorylation
K112 Acetylation
K112 Ubiquitination
K118 Acetylation
K118 Ubiquitination
S123 Phosphorylation
S132 Phosphorylation
K149 Acetylation
R160 Methylation
K161 Acetylation
K161 Methylation
Y162 Phosphorylation
K168 Ubiquitination
S174 Phosphorylation
T199 Phosphorylation
T201 Phosphorylation
S206 Phosphorylation
K213 Ubiquitination
Y214 Phosphorylation
S215 Phosphorylation
Y221 Phosphorylation
K226 Ubiquitination
S229 Phosphorylation
K231 Ubiquitination
K233 Ubiquitination
T237 Phosphorylation
T247 Phosphorylation
K248 Ubiquitination
S252 Phosphorylation
Y261 Phosphorylation
K264 Ubiquitination
Y267 Phosphorylation
K268 Ubiquitination
S271 Phosphorylation
T282 Phosphorylation
S283 Phosphorylation P53355 (DAPK1)

研究背景

功能:

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

翻译修饰:

Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells.

细胞定位:

Cytoplasm>Cytoskeleton.
Note: Associates with F-actin stress fibers.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

亚基结构:

Homodimer. Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain (By similarity). Interacts with HRG (via the HRR domain); the interaction contributes to the antiangiogenic properties of the histidine/proline-rich region (HRR) of HRG (By similarity). Interacts (via N-terminus) with LMOD2 (via N-terminus) and TMOD1 (via N-terminus).

蛋白家族:

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Belongs to the tropomyosin family.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

文献引用

1). Allicin induces cell cycle arrest and apoptosis of breast cancer cells in vitro via modulating the p53 pathway. Molecular Biology Reports, 2021 (PubMed: 34626309) [IF=2.8]
2). miR-22 alleviates sepsis-induced acute kidney injury via targeting the HMGB1/TLR4/NF-κB signaling pathway. INTERNATIONAL UROLOGY AND NEPHROLOGY, 2023 (PubMed: 35960478) [IF=2.0]

Application: WB    Species: Rat    Sample: HBZY-1 cells

Fig. 7 miR-22 decreased the expression of TLR4, TLR2, MyD88, and p-NF-κB p65 protein in the AKI vitro model. Western blot was conducted to measure the protein expression of TLR4, TLR2, MyD88, TM and p-NF-κB p65 in HBZY-1 cells. #p < 0.05, ##p < 0.01 vs. Control. *p < 0.05, **p < 0.01 vs. LPS

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