Profilin 1 Antibody - #DF6298

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产品: Profilin 1 抗体
货号: DF6298
描述: Rabbit polyclonal antibody to Profilin 1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 15kDa; 15kD(Calculated).
蛋白号: P07737
RRID: AB_2838264

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Profilin 1 Antibody detects endogenous levels of total Profilin 1.
RRID:
AB_2838264
引用格式: Affinity Biosciences Cat# DF6298, RRID:AB_2838264.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Actin binding protein; ALS18; Epididymis tissue protein Li 184a; OTTHUMP00000125244; PFN 1; Pfn; PFN1; PROF1_HUMAN; Profilin I; Profilin-1; Profilin1; ProfilinI;

抗原和靶标

免疫原:
Uniprot:

P07737(PROF1_HUMAN) >>Visit HPA database.

基因/基因ID:
PFN1(5216)
表达:
P07737 PROF1_HUMAN:

Expressed in epididymis (at protein level).

描述:
The dynamic polymerization and depolymerization of actin filaments, a process governed by external and internal signaling events, is vital for cell motility (immune cell function, migration, invasion, metastasis, angiogenesis), cell division and adhesion. Among the many regulators of actin dynamics are profilins. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting the exchange of ADP for ATP (reviewed in 1). Profilins bind to proteins involved in the regulation of actin dynamics including palladin (2), dynamin-1 (3), VASP (4) and N-WASP (5). In mice, knockout of the ubiquitously expressed profilin-1 indicates that the protein is essential for embryonic development (6). Profilin-2 is primarily expressed in brain and functions in the regulation of neurite outgrowth (7), membrane trafficking and endocytosis (3). The recently cloned profilin-3 is expressed in kidney and testes (8).
序列:
MAGWNAYIDNLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVNITPAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVTVTKTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
50
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07737 作为底物

Site PTM Type Enzyme
A2 Acetylation
Y7 Phosphorylation
T16 Phosphorylation
Y25 Phosphorylation
K26 Ubiquitination
S28 Phosphorylation
S30 Phosphorylation
K38 Acetylation
K38 Ubiquitination
T39 Phosphorylation
T44 Phosphorylation
K54 Acetylation
K54 Methylation
K54 Ubiquitination
R56 Methylation
S57 Phosphorylation
Y60 Phosphorylation
T65 Phosphorylation
K70 Ubiquitination
S72 Phosphorylation
S77 Phosphorylation
S85 Phosphorylation
T90 Phosphorylation
K91 Acetylation
K91 Ubiquitination
S92 Phosphorylation
T93 Phosphorylation
T98 Phosphorylation
T102 Phosphorylation
T104 Phosphorylation
K105 Acetylation
K105 Ubiquitination
T106 Phosphorylation
K108 Acetylation
K108 Ubiquitination
T109 Phosphorylation
K116 Ubiquitination
K126 Acetylation
K126 Ubiquitination
K127 Ubiquitination
C128 S-Nitrosylation
Y129 Phosphorylation P12931 (SRC) , P35968 (KDR)
S133 Phosphorylation
S138 Phosphorylation Q13464 (ROCK1)
Y140 Phosphorylation

研究背景

功能:

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

翻译修饰:

Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.

细胞定位:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in epididymis (at protein level).

亚基结构:

Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with VASP. Interacts with HTT.

蛋白家族:

Belongs to the profilin family.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

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