RHEB Antibody - #DF6299

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产品: RHEB 抗体
货号: DF6299
描述: Rabbit polyclonal antibody to RHEB
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 20kDa; 20kD(Calculated).
蛋白号: Q15382
RRID: AB_2838265

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)
克隆:
Polyclonal
特异性:
RHEB Antibody detects endogenous levels of total RHEB.
RRID:
AB_2838265
引用格式: Affinity Biosciences Cat# DF6299, RRID:AB_2838265.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Ras homolog enriched in brain 2, formerly; GTP binding protein Rheb; GTP-binding protein Rheb; MGC111559; Ras homolog enriched in brain 2; Ras homolog enriched in brain; RHEB 2; Rheb; RHEB_HUMAN; RHEB2; RHEB2, formerly;

抗原和靶标

免疫原:
Uniprot:

Q15382(RHEB_HUMAN) >>Visit HPA database.

基因/基因ID:
RHEB(6009)
表达:
Q15382 RHEB_HUMAN:

Ubiquitous. Highest levels observed in skeletal and cardiac muscle.

描述:
Rheb (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Like other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/TOR signaling pathway (3-6). The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting Rheb via tuberin's GAP activity (7).
序列:
MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Xenopus
83
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15382 作为底物

Site PTM Type Enzyme
Y14 Phosphorylation
S16 Phosphorylation
K102 Ubiquitination
K109 Ubiquitination
K120 Ubiquitination
K121 Ubiquitination
S130 Phosphorylation Q8IW41 (MAPKAPK5)
K135 Ubiquitination
S140 Phosphorylation
K151 Ubiquitination
K169 Ubiquitination
S175 Phosphorylation

翻译修饰 - Q15382 作为激酶

Substrate Site Source
P04049-1 (RAF1) S338 Uniprot
P15056 (BRAF) S446 Uniprot

研究背景

功能:

Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.

翻译修饰:

Farnesylation is important for efficiently activating mTORC1-mediated signaling.

Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.

细胞定位:

Endomembrane system>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane>Lipid-anchor>Cytoplasmic side. Cytoplasm>Cytosol. Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Highest levels observed in skeletal and cardiac muscle.

亚基结构:

Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Interacts (when prenylated) with PDE6D; this promotes release from membranes.

蛋白家族:

Belongs to the small GTPase superfamily. Rheb family.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). An Injectable Sustained Release Hydrogel of Hyaluronic Acid Loaded with β-Ecdysterone Ameliorates Cartilage Damage in Osteoarthritis via Activating Autophagy. Advanced Therapeutics, 2023 [IF=4.6]

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