CAPN1 Antibody - #DF6306

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产品: CAPN1 抗体
货号: DF6306
描述: Rabbit polyclonal antibody to CAPN1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 82kDa; 82kD(Calculated).
蛋白号: P07384
RRID: AB_2838272

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
CAPN1 Antibody detects endogenous levels of total CAPN1.
RRID:
AB_2838272
引用格式: Affinity Biosciences Cat# DF6306, RRID:AB_2838272.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Ca2 activated neutral protease; Calcium activated neutral proteinase; Calcium activated neutral proteinase small subunit; Calcium dependent protease small subunit 1; Calcium dependent protease small subunit; Calcium-activated neutral proteinase 1; Calpain 1; Calpain 1 large subunit; Calpain 1, (mu/I) large subunit; Calpain mu type; Calpain mu-type; Calpain regulatory subunit; Calpain small subunit 1; Calpain, large polypeptide L1; Calpain-1 catalytic subunit; Calpain-1 large subunit; CAN1_HUMAN; CANP 1; CANP; CANP small subunit; CANP1; CANPL 1; CANPL1; CAPN 1; CAPN1; Cell proliferation inducing protein 30; Cell proliferation-inducing gene 30 protein; Micromolar Calpain; Micromolar-calpain; Mu Calpain; muCANP; muCL;

抗原和靶标

免疫原:
Uniprot:

P07384(CAN1_HUMAN) >>Visit HPA database.

基因/基因ID:
CAPN1(823)
表达:
P07384 CAN1_HUMAN:

Ubiquitous.

描述:
Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation (1).
序列:
MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07384 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K20 Ubiquitination
K36 Ubiquitination
Y42 Phosphorylation
K71 Ubiquitination
S77 Phosphorylation
S78 Phosphorylation
K79 Ubiquitination
T80 Phosphorylation
Y81 Phosphorylation
K84 Acetylation
K84 Ubiquitination
K86 Ubiquitination
S93 Phosphorylation
K193 Ubiquitination
K229 Methylation
K229 Ubiquitination
S232 Phosphorylation
Y235 Phosphorylation
K240 Ubiquitination
S251 Phosphorylation
S255 Phosphorylation
S256 Phosphorylation
K270 Ubiquitination
K280 Ubiquitination
S290 Phosphorylation
K328 Ubiquitination
T354 Phosphorylation
K359 Ubiquitination
S360 Phosphorylation
T362 Phosphorylation
K365 Ubiquitination
Y371 Phosphorylation
T374 Phosphorylation
S379 Phosphorylation
T380 Phosphorylation
Y387 Phosphorylation
K398 Ubiquitination
T404 Phosphorylation
Y410 Phosphorylation
S415 Phosphorylation
S418 Phosphorylation
Y446 Phosphorylation
K461 Ubiquitination
S470 Phosphorylation
S474 Phosphorylation
Y494 Phosphorylation
K505 Ubiquitination
S539 Phosphorylation
K564 Ubiquitination
K583 Ubiquitination
S622 Phosphorylation
S636 Phosphorylation

研究背景

功能:

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

翻译修饰:

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.

细胞定位:

Cytoplasm. Cell membrane.
Note: Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Forms a heterodimer with a small (regulatory) subunit CAPNS1.

蛋白家族:

Belongs to the peptidase C2 family.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

文献引用

1). Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain Signaling. Biological trace element research, 2020 (PubMed: 31797207) [IF=3.9]

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