FAAH Antibody - #DF6308

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产品: FAAH 抗体
货号: DF6308
描述: Rabbit polyclonal antibody to FAAH
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Horse, Rabbit, Dog
分子量: 63kDa; 63kD(Calculated).
蛋白号: O00519
RRID: AB_2838274

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(88%), Horse(100%), Rabbit(100%), Dog(89%)
克隆:
Polyclonal
特异性:
FAAH Antibody detects endogenous levels of total FAAH.
RRID:
AB_2838274
引用格式: Affinity Biosciences Cat# DF6308, RRID:AB_2838274.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Anandamide amidohydrolase 1; Anandamide amidohydrolase; FA2H; FAAH; FAAH-1; FAAH1_HUMAN; Fatty acid 2-hydroxylase; Fatty acid alpha-hydroxylase; Fatty acid amide hydrolase; Fatty-acid amide hydrolase 1; MGC102823; MGC138146; Oleamide hydrolase 1; Oleamide hydrolase;

抗原和靶标

免疫原:
Uniprot:

O00519(FAAH1_HUMAN) >>Visit HPA database.

基因/基因ID:
FAAH(2166)
表达:
O00519 FAAH1_HUMAN:

Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.

描述:
Endogenous cannabinoids have been implicated in addictive behaviors and drug abuse (1). Fatty-acid amide hydrolase 1 (FAAH1) is a plasma membrane-bound hydrolase that converts oleamide to oleic acid (2). This hydrolase also converts the cannabinoid anandamide, the endogenous ligand for the CB1 cannabinoid receptor, to arachidonic acid, suggesting a role in fatty-acid amide inactivation (2). Mice lacking FAAH1 have significantly higher levels of anandamide in the brain and show decreased sensitivity to pain, further indicating a role for FAAH1 in the regulation of endocannabinoid signaling in vivo (3). FAAH1 null mice also demonstrate an increased preference for alcohol and an increased voluntary uptake of alcohol as compared to wild-type mice, indicating a role of FAAH1 in modulating addictive behaviors (1).
序列:
MVQYELWAALPGASGVALACCFVAAAVALRWSGRRTARGAVVRARQRQRAGLENMDRAAQRFRLQNPDLDSEALLALPLPQLVQKLHSRELAPEAVLFTYVGKAWEVNKGTNCVTSYLADCETQLSQAPRQGLLYGVPVSLKECFTYKGQDSTLGLSLNEGVPAECDSVVVHVLKLQGAVPFVHTNVPQSMFSYDCSNPLFGQTVNPWKSSKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSSFCGICGLKPTGNRLSKSGLKGCVYGQEAVRLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEVYTSSQPLRVGYYETDNYTMPSPAMRRAVLETKQSLEAAGHTLVPFLPSNIPHALETLSTGGLFSDGGHTFLQNFKGDFVDPCLGDLVSILKLPQWLKGLLAFLVKPLLPRLSAFLSNMKSRSAGKLWELQHEIEVYRKTVIAQWRALDLDVVLTPMLAPALDLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Horse
100
Dog
89
Pig
88
Bovine
67
Sheep
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00519 作为底物

Site PTM Type Enzyme
S88 Phosphorylation
T99 Phosphorylation
S241 Phosphorylation
S289 Phosphorylation
Y329 Phosphorylation
Y335 Phosphorylation
K423 Ubiquitination
S430 Phosphorylation
S434 Phosphorylation
S440 Phosphorylation
Y454 Phosphorylation

研究背景

功能:

Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.

细胞定位:

Endomembrane system>Single-pass membrane protein. Cytoplasm>Cytoskeleton.
Note: Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the amidase family.

研究领域

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

文献引用

1). MicroRNA miR-1275 coordinately regulates AEA/LPA signals via targeting FAAH in lipid metabolism reprogramming of gastric cancer. Cell Death & Disease, 2023 (PubMed: 36702852) [IF=9.0]

Application: WB    Species: Mouse    Sample: GC tissues

Fig. 1: FAAH is highly expressed in GC tissues.A Clustered heatmap. Each row represents a tissue sample, and each column represents a coding gene. B Volcano plots. The red points on the right indicate the differentially upregulated genes with statistical significance while the red points on the left indicate the downregulated genes. Differential expression of FAAH in tissues from C TCGA database and D Oncomine database. E Differential expression analysis of FAAH in 4 GC subtypes (data from TCGA). F mRNA levels of FAAH in 96 pairs of GC tissues. G Protein levels of FAAH in 20 pairs of GC tissues. *p 

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