| 产品: | FEN1 抗体 |
| 货号: | DF6309 |
| 描述: | Rabbit polyclonal antibody to FEN1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 43kDa; 43kD(Calculated). |
| 蛋白号: | P39748 |
| RRID: | AB_2838275 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6309, RRID:AB_2838275.
展开/折叠
DNase IV; FEN-1; FEN1; FEN1_HUMAN; Flap endonuclease 1; Flap structure specific endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2;
抗原和靶标
- P39748 FEN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P39748 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| R19 | Methylation | Uniprot | |
| K24 | Sumoylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| K80 | Acetylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K88 | Sumoylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K93 | Sumoylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| R100 | Methylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| R104 | Methylation | Uniprot | |
| K109 | Acetylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K125 | Acetylation | Uniprot | |
| K125 | Sumoylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K128 | Sumoylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| C163 | S-Nitrosylation | Uniprot | |
| K168 | Sumoylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| S187 | Phosphorylation | P06493 (CDK1) | Uniprot |
| R192 | Methylation | Uniprot | |
| T195 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S197 | Phosphorylation | Uniprot | |
| K200 | Acetylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K201 | Acetylation | Uniprot | |
| K201 | Sumoylation | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K254 | Sumoylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| K267 | Acetylation | Uniprot | |
| K267 | Sumoylation | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| Y268 | Phosphorylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K297 | Sumoylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K308 | Sumoylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K314 | Sumoylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| K345 | Methylation | Uniprot | |
| K345 | Sumoylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| K354 | Acetylation | Uniprot | |
| K354 | Sumoylation | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| K375 | Acetylation | Uniprot | |
| K375 | Sumoylation | Uniprot | |
| K377 | Acetylation | Uniprot | |
| K380 | Acetylation | Uniprot |
研究背景
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
Nucleus>Nucleolus. Nucleus>Nucleoplasm.
Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
Mitochondrion.
Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300; can bind simultaneously to both PCNA and EP300. Interacts with PCNA; can bind simultaneously to both PCNA and EP300. Interacts with DDX11; this interaction is direct and increases flap endonuclease activity of FEN1. Interacts with WDR4; regulating its endonuclease activity.
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
研究领域
· Genetic Information Processing > Replication and repair > DNA replication.
· Genetic Information Processing > Replication and repair > Base excision repair.
· Genetic Information Processing > Replication and repair > Non-homologous end-joining.
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