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Western blot analysis of Hela whole cell lysates, using FEN1 Antibody.

DF6309 at 1/100 staining Rat lung tissue by IHC-P.

产品:	FEN1 抗体
货号:	DF6309
描述:	Rabbit polyclonal antibody to FEN1
应用:	WB IHC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量:	43kDa; 43kD(Calculated).
蛋白号:	P39748
RRID:	AB_2838275

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

克隆:

Polyclonal

特异性:

FEN1 Antibody detects endogenous levels of total FEN1.

RRID:

AB_2838275
引用格式: Affinity Biosciences Cat# DF6309, RRID:AB_2838275.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

DNase IV; FEN-1; FEN1; FEN1_HUMAN; Flap endonuclease 1; Flap structure specific endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2;

抗原和靶标

免疫原:

Uniprot:

P39748(FEN1_HUMAN) >>Visit HPA database.

基因/基因ID:

FEN1(2237)

描述:

Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10).

序列:

P39748 FEN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P39748 作为底物

P39748

Site	PTM Type	Enzyme	Source
K8	Ubiquitination		Uniprot
S16	Phosphorylation		Uniprot
R19	Methylation		Uniprot
K24	Sumoylation		Uniprot
K24	Ubiquitination		Uniprot
K80	Acetylation		Uniprot
K80	Ubiquitination		Uniprot
K88	Sumoylation		Uniprot
K88	Ubiquitination		Uniprot
K93	Sumoylation		Uniprot
K99	Ubiquitination		Uniprot
R100	Methylation		Uniprot
S101	Phosphorylation		Uniprot
R104	Methylation		Uniprot
K109	Acetylation		Uniprot
K109	Ubiquitination		Uniprot
K125	Acetylation		Uniprot
K125	Sumoylation		Uniprot
K125	Ubiquitination		Uniprot
K128	Sumoylation		Uniprot
K128	Ubiquitination		Uniprot
K132	Ubiquitination		Uniprot
K135	Ubiquitination		Uniprot
K142	Ubiquitination		Uniprot
C163	S-Nitrosylation		Uniprot
K168	Sumoylation		Uniprot
K168	Ubiquitination		Uniprot
K171	Ubiquitination		Uniprot
S187	Phosphorylation	P06493 (CDK1)	Uniprot
R192	Methylation		Uniprot
T195	Phosphorylation	O14757 (CHEK1)	Uniprot
S197	Phosphorylation		Uniprot
K200	Acetylation		Uniprot
K200	Ubiquitination		Uniprot
K201	Acetylation		Uniprot
K201	Sumoylation		Uniprot
K201	Ubiquitination		Uniprot
Y234	Phosphorylation		Uniprot
K252	Ubiquitination		Uniprot
K254	Sumoylation		Uniprot
K254	Ubiquitination		Uniprot
S255	Phosphorylation		Uniprot
K267	Acetylation		Uniprot
K267	Sumoylation		Uniprot
K267	Ubiquitination		Uniprot
Y268	Phosphorylation		Uniprot
K277	Ubiquitination		Uniprot
S293	Phosphorylation		Uniprot
K297	Sumoylation		Uniprot
K297	Ubiquitination		Uniprot
K308	Sumoylation		Uniprot
K308	Ubiquitination		Uniprot
K314	Sumoylation		Uniprot
K314	Ubiquitination		Uniprot
S317	Phosphorylation		Uniprot
S329	Phosphorylation		Uniprot
S331	Phosphorylation		Uniprot
S335	Phosphorylation		Uniprot
T336	Phosphorylation		Uniprot
K345	Methylation		Uniprot
K345	Sumoylation		Uniprot
K345	Ubiquitination		Uniprot
S349	Phosphorylation		Uniprot
S351	Phosphorylation		Uniprot
S352	Phosphorylation		Uniprot
K354	Acetylation		Uniprot
K354	Sumoylation		Uniprot
K354	Ubiquitination		Uniprot
S363	Phosphorylation		Uniprot
T364	Phosphorylation		Uniprot
K375	Acetylation		Uniprot
K375	Sumoylation		Uniprot
K377	Acetylation		Uniprot
K380	Acetylation		Uniprot

研究背景

P39748_FEN1_HUMAN

功能:

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

翻译修饰:

Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.

Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.

Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.

细胞定位:

Nucleus>Nucleolus. Nucleus>Nucleoplasm.
Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.

Mitochondrion.

亚基结构:

Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300; can bind simultaneously to both PCNA and EP300. Interacts with PCNA; can bind simultaneously to both PCNA and EP300. Interacts with DDX11; this interaction is direct and increases flap endonuclease activity of FEN1. Interacts with WDR4; regulating its endonuclease activity.

蛋白家族:

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

研究领域

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Base excision repair.

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

文献引用

1). ALKBH5-mediated m 6 A modification of CDC6 mRNA impairs gastric cancer tumorigenesis and has prognostic significance. Research Square, 2023

Application: WB Species: Human Sample: MKN-28 cells

Figure 3. RNA-seq analysis of the enrichment of ALKBH5 in GC proliferation and cycle progression. (A) RNA-seq and volcano plotting of the DEGs between ALKBH5 and control groups and their distribution on human chromosomes. (B) KEGG analysis of the enrichment pathways by the upregulated or downregulated genes caused by ALKBH5-OE in GC cells. (C) GO analysis of the enriched biological function of the upregulated or downregulated genes caused by ALKBH5-OE in GC cells. (D) Venn analysis of the intersection of Cell cycle and DNA replication-related genes (n = 147) and DEGs from the KEGG dataset (n = 159). (E) Bubble chart displaying the expression levels of cell proliferation and cycle-related 7 target genes regulated by ALKIBH5 in MKN-28 cells. (F) Western blotting analysis of the effects of ALKBH5 overexpression on the protein levels of 7 target genes in MKN-28 cells. (G) TCGA and (H) GEPIA databases uncovering the expression levels of 7 target genes regulated by ALKBH5 in GC tissue samples.

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FEN1 Antibody - #DF6309

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