SERPINB5 Antibody - #DF6313

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产品: SERPINB5 抗体
货号: DF6313
描述: Rabbit polyclonal antibody to SERPINB5
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 42kDa; 42kD(Calculated).
蛋白号: P36952
RRID: AB_2838279

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 50ul RMB¥ 1250 现货
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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%)
克隆:
Polyclonal
特异性:
SERPINB5 Antibody detects endogenous levels of total SERPINB5.
RRID:
AB_2838279
引用格式: Affinity Biosciences Cat# DF6313, RRID:AB_2838279.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Maspin; Ovalbumin; Peptidase inhibitor 5; PI-5; PI5; protease inhibitor 5 (maspin); Protease inhibitor 5; Serine (or cysteine) peptidase inhibitor, clade B, member 5; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin family B member 5; Serpin peptidase inhibitor; Serpin peptidase inhibitor, clade B (ovalbumin), member 5; Serpinb5; SPB5_HUMAN; Spi5; Spi7;

抗原和靶标

免疫原:
Uniprot:

P36952(SPB5_HUMAN) >>Visit HPA database.

基因/基因ID:
SERPINB5(5268)
表达:
P36952 SPB5_HUMAN:

Normal mammary epithelial cells.

描述:
Maspin (SERPINB5) was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines (1). While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor (2). It plays an essential role in embryonic development through critical roles in cell adhesion (3). While the precise mechanism of maspin signaling is unclear (4), the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis (5,6) and angiogenesis (7), while promoting apoptosis (8). Nuclear translocation of active IKKα has been shown to repress maspin transcription and promote prostate cancer metastasis (9).
序列:
MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFSESETKECPFRVNKTDTKPVQMMNMEATFCMGNIDSINCKIIELPFQNKHLSMFILLPKDVEDESTGLEKIEKQLNSESLSQWTNPSTMANAKVKLSIPKFKVEKMIDPKACLENLGLKHIFSEDTSDFSGMSETKGVALSNVIHKVCLEITEDGGDSIEVPGARILQHKDELNADHPFIYIIRHNKTRNIIFFGKFCSP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
91
Chicken
64
Xenopus
45
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P36952 作为底物

Site PTM Type Enzyme
S9 Phosphorylation
T50 Phosphorylation
K64 Ubiquitination
K79 Ubiquitination
S85 Phosphorylation
Y93 Phosphorylation
S97 Phosphorylation
K109 Ubiquitination
K114 Acetylation
K114 Ubiquitination
T118 Phosphorylation
K129 Ubiquitination
S135 Phosphorylation
K137 Ubiquitination
T157 Phosphorylation
S240 Phosphorylation
T241 Phosphorylation
K245 Ubiquitination
K248 Ubiquitination
K275 Ubiquitination
K277 Acetylation
K280 Acetylation
K285 Ubiquitination
S298 Phosphorylation
S302 Phosphorylation
T310 Phosphorylation
K311 Ubiquitination
S316 Phosphorylation
K345 Ubiquitination
Y356 Phosphorylation

研究背景

功能:

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

细胞定位:

Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Normal mammary epithelial cells.

亚基结构:

Interacts with IRF6.

蛋白家族:

Belongs to the serpin family. Ov-serpin subfamily.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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