产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6320, RRID:AB_2838286.
展开/折叠
1200007E24Rik; ADP ribosylation factor binding protein 2; ADP ribosylation factor binding protein GGA 2; ADP ribosylation factor binding protein GGA2; ADP-ribosylation factor-binding protein GGA2; ARF-binding protein 2; FLJ20966; Gamma adaptin related protein 2; gamma ear-containing; Gamma-adaptin-related protein 2; GGA 2; Gga2; GGA2_HUMAN; Golgi associated gamma adaptin ear containing ARF binding protein 2; Golgi localized gamma ear containing ARF binding protein 2; Golgi-localized; KIAA1080; Vear; VHS domain and ear domain containing protein; VHS domain and ear domain of gamma adaptin; VHS domain and ear domain of gamma-adaptin;
抗原和靶标
- Q9UJY4 GGA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAATAVAAAVAGTESAQGPPGPAASLELWLNKATDPSMSEQDWSAIQNFCEQVNTDPNGPTHAPWLLAHKIQSPQEKEALYALTVLEMCMNHCGEKFHSEVAKFRFLNELIKVLSPKYLGSWATGKVKGRVIEILFSWTVWFPEDIKIRDAYQMLKKQGIIKQDPKLPVDKILPPPSPWPKSSIFDADEEKSKLLTRLLKSNHPEDLQAANRLIKNLVKEEQEKSEKVSKRVSAVEEVRSHVKVLQEMLSMYRRPGQAPPDQEALQVVYERCEKLRPTLFRLASDTTDDDDALAEILQANDLLTQGVLLYKQVMEGRVTFGNRVTSSLGDIPVSRVFQNPAGCMKTCPLIDLEVDNGPAQMGTVVPSLLHQDLAALGISDAPVTGMVSGQNCCEEKRNPSSSTLPGGGVQNPSADRNLLDLLSAQPAPCPLNYVSQKSVPKEVPPGTKSSPGWSWEAGPLAPSPSSQNTPLAQVFVPLESVKPSSLPPLIVYDRNGFRILLHFSQTGAPGHPEVQVLLLTMMSTAPQPVWDIMFQVAVPKSMRVKLQPASSSKLPAFSPLMPPAVISQMLLLDNPHKEPIRLRYKLTFNQGGQPFSEVGEVKDFPDLAVLGAA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UJY4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K103 | Ubiquitination | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K219 | Sumoylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| T278 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| T447 | Phosphorylation | Uniprot | |
| K545 | Ubiquitination | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| K602 | Ubiquitination | Uniprot |
研究背景
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Regulates retrograde transport of phosphorylated form of BACE1 from endosomes to the trans-Golgi network.
Ubiquitinated.
Golgi apparatus>trans-Golgi network membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein. Early endosome membrane>Peripheral membrane protein.
Ubiquitously expressed.
Monomer (Probable). Interacts with NECAP1, TSG101, UBC and AFTPH/aftiphilin. Interacts with CNST (By similarity). Interacts with GGA1 and GGA3. Binds to clathrin and activated ARFs, such as ARF1, ARF5 and ARF6. Binds RABEP1 and RABGEF1. Interacts with the type-I membrane proteins LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. Interacts (via N-terminal VHS domain) with SORL1/sorLA and SORT1 (via C-terminal cytosolic domain). Binds the accessory proteins CCDC91, P200, SYNRG, EPN4 and NECAP2. Interacts with ADRA2B. Interacts (via VHS domain) with PIK4B; the interaction is important for PIK4B location at the Golgi apparatus membrane.
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (DXXLL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
Belongs to the GGA protein family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
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