TPM3 Antibody - #DF6338

Fig. 1. STIM1 and TPM3 Interact and colocalization. A) Five genes are shown having a correlation coefficient with STIM1 greater than 0.7. The X-axis represents the results of each of 80 samples across 11 organs and four age groups; the Y-axis represents the expression levels of the five genes in each sample. Expression levels of TPM3 are highly correlated with those of STIM1, with both proteins highly expressed in numerous tissues and organs. B and C) Representative images of a coimmunoprecipitation (co-IP) experiment. Panel B shows that protein from the mesenteric resistance artery (MRA) is precipitated by a STIM1 antibody and detected by a TPM3 antibody. Panel C shows that protein from the MRA is precipitated by a TPM3 antibody and detected by a STIM1 antibody. Panels B and C also show immunoblots (IB) of cell lysates. D and E) Identification of primary cultured vascular smooth muscle cells (VSMCs). Positive green fluorescence is observed in VSMCs treated with an antibody to smooth muscle α-actin (α-SMA) (D). No immunofluorescence is observed in the negative control (Phosphate Buffered Saline, replaced primary antibody in E). F) Colocalization of STIM1 and TPM3 in VSMCs. Red fluorescence represents TPM3; green fluorescence represents STIM1; blue fluorescence refers nucleus. Merged image indicates STIM1 and TPM3 co-localization. FPKM represents fragments per kilobase of exon model per million reads mapped (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).