产品: ECI1 抗体
货号: DF6343
描述: Rabbit polyclonal antibody to ECI1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 33kDa; 33kD(Calculated).
蛋白号: P42126
RRID: AB_2838307

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(100%), Sheep(89%), Rabbit(92%), Chicken(92%), Xenopus(92%)
克隆:
Polyclonal
特异性:
ECI1 Antibody detects endogenous levels of total ECI1.
RRID:
AB_2838307
引用格式: Affinity Biosciences Cat# DF6343, RRID:AB_2838307.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2-trans-enoyl-CoA isomerase; 3; 3,2 trans enoyl Coenzyme A isomerase; 3,2 trans-enoyl-CoA isomerase; 3,2-trans-enoyl-CoA isomerase, mitochondrial; Acetylene allene isomerase; D2-enoyl-CoA isomerase; D3; D3,D2 enoyl CoA isomerase; DCI; DCI protein; delta(2)-enoyl-CoA isomerase; Delta(3); Delta(3),Delta(2) enoyl CoA isomerase; Delta3, delta2 enoyl CoA isomerase; Dodecenoyl CoA delta Isomerase; Dodecenoyl CoA isomerase; Dodecenoyl Coenzyme A delta isomerase; Dodecenoyl Coenzyme A delta isomerase (3,2 trans enoyl Coenzyme A isomerase); Dodecenoyl Coenzyme A delta isomerase 3,2 trans enoyl Coenzyme A isomerase, isoform CRA a; Dodecenoyl-CoA isomerase; ECI1; ECI1_HUMAN; Enoyl-CoA delta isomerase 1; enoyl-CoA delta isomerase 1, mitochondrial; mitochondrial;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]
序列:
MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVLVSAINGACPAGGCLVALTCDYRILADNPRYCIGLNETQLGIIAPFWLKDTLENTIGHRAAERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKEEKG

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Xenopus
92
Chicken
92
Rabbit
92
Sheep
89
Pig
78
Bovine
67
Dog
67
Zebrafish
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P42126 作为底物

Site PTM Type Enzyme
K61 Ubiquitination
K89 Acetylation
R171 Methylation
S279 Phosphorylation
S282 Phosphorylation
K283 Acetylation
K283 Ubiquitination
S285 Phosphorylation
K288 Acetylation
K288 Ubiquitination

研究背景

功能:

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species.

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homotrimer.

蛋白家族:

Belongs to the enoyl-CoA hydratase/isomerase family.

研究领域

· Metabolism > Lipid metabolism > Fatty acid degradation.

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