产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6346, RRID:AB_2838310.
展开/折叠
ARHGDIA; fa96g11; GDIA 1; GDIA1; GDIR1_HUMAN; MGC117248; NPHS8; Rho GDI 1; Rho GDI alpha; Rho GDI; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP dissociation inhibitor 1; Rho GDP dissociation inhibitor alpha; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha; RhoGDI 1; RhoGDI alpha; RHOGDI; RhoGDI1; wu:fa96g11; zgc:55554; zgc:77681;
抗原和靶标
- P52565 GDIR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVVVTGLTLVCSSAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPVEEAPKGMLARGSYSIKSRFTDDDKTDHLSWEWNLTIKKDWKD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P52565 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| Y27 | Phosphorylation | P12931 (SRC) | Uniprot |
| K28 | Acetylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| S34 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K43 | Acetylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| Y51 | Phosphorylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| K52 | Methylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| S96 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K99 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| K105 | Acetylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| T132 | Phosphorylation | Uniprot | |
| Y133 | Phosphorylation | Uniprot | |
| K138 | Sumoylation | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Sumoylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| Y156 | Phosphorylation | P12931 (SRC) | Uniprot |
| T160 | Phosphorylation | Uniprot | |
| K167 | Acetylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S174 | Phosphorylation | P17612 (PRKACA) , Q13153 (PAK1) | Uniprot |
| K178 | Acetylation | Uniprot | |
| K178 | Ubiquitination | Uniprot |
研究背景
Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
Cytoplasm.
Monomer (By similarity). Interacts with FER. Interacts with PLXNB3 (By similarity). Forms a heterodimer with RAC1. Interacts with RHOA, the affinity is increased by three orders of magnitude when RHOA is prenylated. Interacts with PSMD10; the interaction increases ARHGDIA association with RHOA, leading to ARHGDIA-mediated inactivation of RHOA and ROCK and prolonged AKT activation. Interacts with KANK2; the interaction is direct and may regulate the interaction of ARHGDIA with RHOA, RAC1 and CDC42. Interacts with RHOC. Interacts with CDC42. Interacts with NGFR (via death domain); NGFR binding decreases the affinity for RHOA.
Belongs to the Rho GDI family.
研究领域
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.
限制条款
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