STIP1 Antibody - #DF6351

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产品: STIP1 抗体
货号: DF6351
描述: Rabbit polyclonal antibody to STIP1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 63kDa; 63kD(Calculated).
蛋白号: P31948
RRID: AB_2838315

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(83%), Dog(92%)
克隆:
Polyclonal
特异性:
STIP1 Antibody detects endogenous levels of total STIP1.
RRID:
AB_2838315
引用格式: Affinity Biosciences Cat# DF6351, RRID:AB_2838315.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Epididymis secretory sperm binding protein Li 94n; HEL S 94n; Hop; Hsc70/Hsp90 organizing protein; Hsc70/Hsp90-organizing protein; IEF SSP 3521; NY REN 11 antigen; P60; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; STIP1_HUMAN; Stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; Transformation sensitive protein IEF SSP 3521; Transformation-sensitive protein IEF SSP 3521;

抗原和靶标

免疫原:
Uniprot:

P31948(STIP1_HUMAN) >>Visit HPA database.

基因/基因ID:
STIP1(10963)
描述:
HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5).
序列:
MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
83
Zebrafish
67
Xenopus
56
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P31948 作为底物

Site PTM Type Enzyme
M1 Acetylation
K8 Acetylation
K10 Acetylation
K13 Ubiquitination
S16 Phosphorylation
C26 S-Nitrosylation
K32 Ubiquitination
Y41 Phosphorylation
S42 Phosphorylation
R44 Methylation
Y48 Phosphorylation
Y54 Phosphorylation
Y58 Phosphorylation
C62 S-Nitrosylation
K68 Acetylation
K68 Ubiquitination
K73 Acetylation
K73 Sumoylation
K73 Ubiquitination
K78 Ubiquitination
R87 Methylation
R93 Methylation
Y95 Phosphorylation
K100 Acetylation
K100 Ubiquitination
K109 Ubiquitination
R118 Methylation
K123 Sumoylation
K123 Ubiquitination
K136 Ubiquitination
T145 Phosphorylation
S148 Phosphorylation
Y152 Phosphorylation
K162 Ubiquitination
K169 Ubiquitination
T198 Phosphorylation P06493 (CDK1)
K207 Acetylation
K210 Acetylation
K210 Sumoylation
K210 Ubiquitination
K229 Acetylation
K237 Acetylation
K246 Acetylation
K246 Ubiquitination
K252 Ubiquitination
Y261 Phosphorylation
Y269 Phosphorylation
Y275 Phosphorylation
K284 Acetylation
K284 Ubiquitination
R293 Methylation
K301 Acetylation
K301 Ubiquitination
K312 Acetylation
K312 Methylation
K317 Ubiquitination
K325 Acetylation
K325 Methylation
K325 Ubiquitination
T332 Phosphorylation P06493 (CDK1)
K337 Acetylation
K337 Ubiquitination
K344 Acetylation
K344 Ubiquitination
K347 Ubiquitination
Y354 Phosphorylation
K364 Acetylation
K364 Ubiquitination
K373 Acetylation
K373 Ubiquitination
K381 Acetylation
Y383 Phosphorylation
T384 Phosphorylation
K388 Acetylation
K388 Ubiquitination
K395 Acetylation
C417 S-Nitrosylation
C420 S-Nitrosylation
K429 Acetylation
K429 Ubiquitination
K434 Ubiquitination
K442 Ubiquitination
Y444 Phosphorylation
K446 Acetylation
K446 Ubiquitination
K453 Ubiquitination
C461 S-Nitrosylation
K462 Acetylation
K462 Ubiquitination
Y476 Phosphorylation
S481 Phosphorylation
K486 Ubiquitination
K513 Acetylation
K513 Ubiquitination
K523 Ubiquitination
K530 Acetylation
K530 Ubiquitination
K533 Ubiquitination
R543 Methylation

研究背景

功能:

Acts as a co-chaperone for HSP90AA1. Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Forms a complex with HSPA8/HSC70, HSPCA/HSP-86 and HSPCB/HSP-84 (By similarity). Interacts with PACRG. Interacts with METTL21B. Interacts with HSP90/HSP90AA1; the interaction dissociates the PPP5C:HSP90AA1 interaction. Interacts with FLCN, FNIP1 and FNIP2. Interacts with HSPA8/HSC70 (By similarity). Interacts with HSP90AB1; upon SMYD2-dependent HSP90AB1 methylation.

蛋白家族:

The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.

研究领域

· Human Diseases > Neurodegenerative diseases > Prion diseases.

文献引用

1). Comparative proteomics identify HSP90A, STIP1 and TAGLN‑2 in serum extracellular vesicles as potential circulating biomarkers for human adenomyosis. Experimental and Therapeutic Medicine, 2022 (PubMed: 35495589) [IF=2.7]

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