IL7R Antibody - #DF6362

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产品: IL7R 抗体
货号: DF6362
描述: Rabbit polyclonal antibody to IL7R
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 52~80kDa; 52kD(Calculated).
蛋白号: P16871
RRID: AB_2838326

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
IL7R Antibody detects endogenous levels of total IL7R.
RRID:
AB_2838326
引用格式: Affinity Biosciences Cat# DF6362, RRID:AB_2838326.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD 127; CD127; CD127 antigen; CDw127; IL 7R alpha; IL 7R; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL7R; IL7RA; IL7RA_HUMAN; IL7Ralpha; ILRA; Interleukin 7 receptor alpha chain; Interleukin 7 receptor; Interleukin 7 receptor isoform H5 6; Interleukin-7 receptor subunit alpha;

抗原和靶标

免疫原:

A synthesized peptide derived from human IL7R, corresponding to a region within N-terminal amino acids.

Uniprot:

P16871(IL7RA_HUMAN) >>Visit HPA database.

基因/基因ID:
IL7R(3575)
描述:
The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). [provided by RefSeq, Jul 2008]
序列:
MTILGTTFGMVFSLLQVVSGESGYAQNGDLEDAELDDYSFSCYSQLEVNGSQHSLTCAFEDPDVNITNLEFEICGALVEVKCLNFRKLQEIYFIETKKFLLIGKSNICVKVGEKSLTCKKIDLTTIVKPEAPFDLSVVYREGANDFVVTFNTSHLQKKYVKVLMHDVAYRQEKDENKWTHVNLSSTKLTLLQRKLQPAAMYEIKVRSIPDHYFKGFWSEWSPSYYFRTPEINNSSGEMDPILLTISILSFFSVALLVILACVLWKKRIKPIVWPSLPDHKKTLEHLCKKPRKNLNVSFNPESFLDCQIHRVDDIQARDEVEGFLQDTFPQQLEESEKQRLGGDVQSPNCPSEDVVITPESFGRDSSLTCLAGNVSACDAPILSSSRSLDCRESGKNGPHVYQDLLLSLGTTNSTLPPPFSLQSGILTLNPVAQGQPILTSLGSNQEEAYVTMSSFYQNQ

翻译修饰 - P16871 作为底物

Site PTM Type Enzyme
N49 N-Glycosylation
N65 N-Glycosylation
N151 N-Glycosylation
Y159 Phosphorylation
Y169 Phosphorylation
K292 Ubiquitination
S297 Phosphorylation
K337 Ubiquitination
S346 Phosphorylation
S365 Phosphorylation
S375 Phosphorylation
Y449 Phosphorylation

研究背景

功能:

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

翻译修饰:

N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.

细胞定位:

Cell membrane>Single-pass type I membrane protein.

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.

蛋白家族:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 4 subfamily.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

文献引用

1). Circ_MTM1 knockdown inhibits the progression of HBV-related liver fibrosis via regulating IL7R expression through targeting miR-122-5p. American Journal of Translational Research, 2022 (PubMed: 35559382) [IF=2.2]

Application: WB    Species: Human    Sample: LX-2 cells

Figure 6 Circ_MTM1 targeted miR-122-5p to regulate IL7R expression. HBV-infected LX-2 cells and LX-2 cells with HBV and TGF-β1 co-treatment were transfected with si-NC, si-circ_MTM1, si-circ_MTM1 + anti-miR-NC, or si-circ_MTM1 + anti-miR-122-5p, respectively. A-D. IL7R expression was detected in HBV-infected LX-2 cells and LX-2 cells with HBV and TGF-β1 co-treatment. A-D. *P < 0.05, si-circ_MTM1 group compared with si-NC group; si-circ_MTM1 + anti-miR-122-5p group compared with si-circ_MTM1 + anti-miR-NC group, n = 6. Intergroup comparisons were analyzed by one-way ANOVA with post hoc Bonferroni test, and the intragroup differences were analyzed by Student’s t-test.

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