| 产品: | Ataxin 3 抗体 |
| 货号: | DF6375 |
| 描述: | Rabbit polyclonal antibody to Ataxin 3 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 43kDa; 41kD(Calculated). |
| 蛋白号: | P54252 |
| RRID: | AB_2838339 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6375, RRID:AB_2838339.
展开/折叠
AT3; Ataxin 3; ataxin 3 variant h; ataxin 3 variant m; ataxin 3 variant ref; Ataxin-3; ATX3; ATX3_HUMAN; ATXN3; EC 3.4.22.; JOS; Josephin; Machado Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3); Machado Joseph disease; Machado Joseph disease protein 1; Machado-Joseph disease protein 1; Machado-Joseph disease protein 1 homolog; MJD; MJD gene; MJD1; Olivopontocerebellar ataxia 3; OTTHUMP00000221583; OTTHUMP00000221585; OTTHUMP00000221586; OTTHUMP00000221587; OTTHUMP00000231995; OTTHUMP00000231997; Rsca3; SCA3; SCA3 gene; Spinocerebellar ataxia type 3 protein;
抗原和靶标
- P54252 ATX3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P54252 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| Y58 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| K117 | Sumoylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K166 | Sumoylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S260 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| K360 | Acetylation | Uniprot |
研究背景
Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension (By similarity). Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription. Regulates autophagy via the deubiquitination of 'Lys-402' of BECN1 leading to the stabilization of BECN1.
Monoubiquitinated N-terminally by UBE2W, possibly leading to activate the deubiquitinating enzyme activity.
Nucleus matrix. Nucleus.
Note: Predominantly nuclear, but not exclusively, inner nuclear matrix.
Ubiquitous.
Interacts with STUB1/CHIP (when monoubiquitinated) (By similarity). Interacts with DNA repair proteins RAD23A and RAD23B. Interacts with BECN1 (via its poly-Gln domain). Interacts with PRKN, UBR2, VCP and tubulin. Short isoform 1 interacts with CASP7.
The UIM domains bind ubiquitin and interact with various E3 ubiquitin-protein ligase, such as STUB1/CHIP. They are essential to limit the length of ubiquitin chains (By similarity).
The poly-Gln domain is involved in the interaction with BECN1 and subsequent starvation-induced autophagy (PubMed:28445460).
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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