产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6377, RRID:AB_2838340.
展开/折叠
HC8; Macropain subunit C8; MGC12306; MGC32631; Multicatalytic endopeptidase complex subunit C8; Proteasome (prosome macropain) subunit alpha type 3; Proteasome alpha 3 subunit; Proteasome component C8; Proteasome subunit alpha type 3; Proteasome subunit alpha type-3; Proteasome subunit C8; PSA3_HUMAN; PSC8; psmA3;
抗原和靶标
- P25788 PSA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDESDDDNM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P25788 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| Y8 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| T36 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K57 | Acetylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K65 | Acetylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | Uniprot | |
| K110 | Acetylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K230 | Acetylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot |
研究背景
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
Cytoplasm. Nucleus.
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Interacts with AURKB. Interacts with CDKN1A. Interacts with MDM2 and RB1. Interacts with the C-terminus of TBXA2R isoform 2. Interacts with DNAJB2.
(Microbial infection) Interacts with HIV-1 Tat protein.
(Microbial infection) Interacts with hepatitis C virus (HCV) F protein.
(Microbial infection) Interacts with Epstein-Barr virus EBNA3 proteins.
Belongs to the peptidase T1A family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
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