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| 产品: | EHMT2 抗体 |
| 货号: | DF6379 |
| 描述: | Rabbit polyclonal antibody to EHMT2 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 132kDa; 132kD(Calculated). |
| 蛋白号: | Q96KQ7 |
| RRID: | AB_2838342 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6379, RRID:AB_2838342.
展开/折叠
Ankyrin repeat containing protein; Bat 8; Bat8; C6orf30; DKFZp686H08213; EHMT 2; Ehmt2; EHMT2_HUMAN; Euchromatic histone lysine methyltransferase 2; Euchromatic histone lysine N methyltransferase 2; Euchromatic histone-lysine N-methyltransferase 2; FLJ35547; G 9a; G9 a; G9a protein; G9A; G9A histone methyltransferase; GAT8; H3 K9 HMTase 3; H3-K9-HMTase 3; Histone H3 K9 methyltransferase 3; Histone H3 K9 methyltransferase3; Histone H3-K9 methyltransferase 3; Histone lysine N methyltransferase; Histone lysine N methyltransferase EHMT2; Histone lysine N methyltransferase, H3 lysine 9 specific 3; Histone lysine N methyltransferase, H3 lysine 9 specific3; Histone-lysine N-methyltransferase EHMT2; HLA B associated transcript 8; HLA-B-associated transcript 8; KMT 1C; KMT1 C; Lysine N methyltransferase 1C; Lysine N-methyltransferase 1C; NG 36; NG36; Protein G9a;
抗原和靶标
Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
- Q96KQ7 EHMT2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAGAAAAAAAEGEAPAEMGALLLEKETRGATERVHGSLGDTPRSEETLPKATPDSLEPAGPSSPASVTVTVGDEGADTPVGATPLIGDESENLEGDGDLRGGRILLGHATKSFPSSPSKGGSCPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAAAAGSEPPPATTSPEGQPKVHRARKTMSKPGNGQPPVPEKRPPEIQHFRMSDDVHSLGKVTSDLAKRRKLNSGGGLSEELGSARRSGEVTLTKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALTEQLSEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSRVALMVLCETHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPAPPPLSQDVPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPLGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELGSLPPVNT
翻译修饰 - Q96KQ7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| T50 | Phosphorylation | Uniprot | |
| K114 | Methylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K122 | Methylation | Uniprot | |
| K131 | Methylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| R184 | Methylation | Uniprot | |
| K185 | Methylation | Uniprot | |
| K189 | Methylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| K445 | Methylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| T560 | Phosphorylation | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| K634 | Ubiquitination | Uniprot | |
| K659 | Ubiquitination | Uniprot | |
| K685 | Ubiquitination | Uniprot | |
| K747 | Ubiquitination | Uniprot | |
| K882 | Ubiquitination | Uniprot | |
| K920 | Ubiquitination | Uniprot | |
| K998 | Ubiquitination | Uniprot | |
| K1047 | Ubiquitination | Uniprot | |
| Y1103 | Phosphorylation | Uniprot | |
| Y1104 | Phosphorylation | Uniprot | |
| S1108 | Phosphorylation | Uniprot | |
| K1164 | Ubiquitination | Uniprot | |
| S1187 | Phosphorylation | Uniprot | |
| S1204 | Phosphorylation | Uniprot | |
| T1210 | Phosphorylation | Uniprot |
研究背景
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
Methylated at Lys-185; automethylated.
Nucleus. Chromosome.
Note: Associates with euchromatic regions. Does not associate with heterochromatin.
Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
Heterodimer; heterodimerizes with EHMT1/GLP. Interacts with GFI1B and WIZ. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with UHRF1. Interacts with CDYL. Interacts with REST only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2. Interacts with PRDM9 and CDYL; interaction only takes place when PRDM9 is bound to hotspot DNA (By similarity).
The SET domain mediates interaction with WIZ.
The ANK repeats bind H3K9me1 and H3K9me2.
In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
研究领域
· Metabolism > Amino acid metabolism > Lysine degradation.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
文献引用
Application: IF/ICC Species: Mouse Sample: MTEC cells
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