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产品:	HO-1 抗体
货号:	DF6391
描述:	Rabbit polyclonal antibody to HO-1
应用:	WB IHC
反应:	Human, Mouse, Rat
分子量:	33kDa; 33kD(Calculated).
蛋白号:	P09601
RRID:	AB_2838354

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封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

克隆:

Polyclonal

特异性:

HO-1 Antibody detects endogenous levels of total HO-1.

RRID:

AB_2838354
引用格式: Affinity Biosciences Cat# DF6391, RRID:AB_2838354.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

32 kD; bK286B10; D8Wsu38e; heat shock protein 32 kD; heat shock protein 32kD; Heat shock protein; Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; Hmox1; HMOX1_HUMAN; HO 1; HO; HO-1; HO1; Hsp32;

抗原和靶标

免疫原:

Uniprot:

P09601(HMOX1_HUMAN) >>Visit HPA database.

基因/基因ID:

HMOX1(3162)

表达:

P09601 HMOX1_HUMAN:

Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

描述:

Hemeoxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron, and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory and cytoprotective functions (2). Hemeoxygenase comprises two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals, and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer disease, endotoxic shock, diabetes, inflammation, and neurological disorders (1,5).

序列:

P09601 HMOX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM

翻译修饰 - P09601 作为底物

P09601

Site	PTM Type	Enzyme	Source
K18	Acetylation		Uniprot
K18	Ubiquitination		Uniprot
K22	Ubiquitination		Uniprot
K39	Acetylation		Uniprot
K39	Ubiquitination		Uniprot
S53	Phosphorylation		Uniprot
Y55	Phosphorylation		Uniprot
Y58	Phosphorylation		Uniprot
K69	Ubiquitination		Uniprot
K86	Ubiquitination		Uniprot
T108	Phosphorylation		Uniprot
Y114	Phosphorylation		Uniprot
Y137	Phosphorylation		Uniprot
K148	Ubiquitination		Uniprot
K149	Ubiquitination		Uniprot
K153	Ubiquitination		Uniprot
S160	Phosphorylation		Uniprot
K177	Ubiquitination		Uniprot
K179	Ubiquitination		Uniprot
S188	Phosphorylation	P31749 (AKT1)	Uniprot
T192	Phosphorylation		Uniprot
S229	Phosphorylation		Uniprot
S231	Phosphorylation		Uniprot
K243	Acetylation		Uniprot
K243	Ubiquitination		Uniprot
T252	Phosphorylation		Uniprot
K256	Acetylation		Uniprot
K256	Ubiquitination		Uniprot
Y286	Phosphorylation		Uniprot

研究背景

P09601_HMOX1_HUMAN

功能:

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

细胞定位:

Microsome. Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.

组织特异性:

Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

蛋白家族:

Belongs to the heme oxygenase family.

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Digestive system > Mineral absorption.

文献引用

1). Tetrahydrocurcumin protects against nonalcoholic fatty liver disease by improving lipid metabolism and redox homeostasis. Journal of Functional Foods, 2022 [IF=5.6]

Application: WB Species: Human Sample: HepG2 cells

Fig. 6. Effect of tetrahydrocurcumin (THC) on lipid content and expression of the NRF2 pathway in HepG2 cells. HepG2 cells were treated with 0.2 mM sodium oleate (OA) for 24 h, and then with THC for 48 h. Oil red O was used to determine the (A) Intracellular lipid content. RT-qPCR and Western blot were used to analyze the expression of NRF2 signaling pathway. (B) Relative expression of NRF2, NQO1 and HO-1 mRNA; (C) Relative expression of GSTA1, GCLC and GCLM mRNA; (D) Relative expression of NRF2 protein; (E) Relative expression of NQO1 protein; (F) Relative expression of HO-1 protein. Different letters (a-d) between groups indicate significant differences

2). Attenuation of blue light-induced photo-oxidative stress through inhibition of NF-κB and MAPK signaling pathways, and activation of Nrf2 signaling pathway by djulis and its bioactive compounds. Journal of Functional Foods, 2023

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HO-1 Antibody - #DF6391

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