NPY Antibody - #DF6431

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(4)   (5)  
产品: NPY 抗体
货号: DF6431
描述: Rabbit polyclonal antibody to NPY
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 11kDa; 11kD(Calculated).
蛋白号: P01303
RRID: AB_2838394

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
NPY Antibody detects endogenous levels of total NPY.
RRID:
AB_2838394
引用格式: Affinity Biosciences Cat# DF6431, RRID:AB_2838394.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C-flanking peptide of NPY; CPON; Neuropeptide tyrosine; Neuropeptide Y precursor; NPY; NPY_HUMAN; Pro neuropeptide Y; PYY 4; PYY4; Y Neuropeptide;

抗原和靶标

免疫原:
Uniprot:

P01303(NPY_HUMAN) >>Visit HPA database.

基因/基因ID:
NPY(4852)
表达:
P01303 NPY_HUMAN:

One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.

描述:
The NPY hormone family consists of NPY, PP and peptide YY. NPY (neuropeptide Y) is a 36 amino acid protein that consists of a polyproline stretch followed by an amphipathic a-helix. NPY shares a 50% amino acid homology with pancreatic polypeptide gene (PP). NPY is expressed throughout the central and peripheral nervous system, particularly in the deep layers of the cortex and smaller cell bodies in the white matter. NPY interacts with the Y-receptor family of G protein-coupled receptors. NPY interacts with NPY receptor Y1 to increase corticotropin levels and decrease noradrenaline levels in the hypothalamus. Through interactions in the hypothalus, NPY plays important roles in the regulation of energy balance by stimulating food intake. NPY favors energy storage by increasing lipoprotein lipase activity in white adipose tissue. A Leucine 7 to Proline 7 polymorphism has been implicated in an increase in alcohol preference, and decrease in cholesterol metabolism.
序列:
MLGNKRLGLSGLTLALSLLVCLGALAEAYPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRYGKRSSPETLISDLLMRESTENVPRTRLEDPAMW

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P01303 作为底物

Site PTM Type Enzyme
Y48 Phosphorylation
Y49 Phosphorylation
S50 Phosphorylation
Y55 Phosphorylation
S75 Phosphorylation
S82 Phosphorylation
T83 Phosphorylation

研究背景

功能:

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

翻译修饰:

The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

细胞定位:

Secreted. Cytoplasmic vesicle>Secretory vesicle>Neuronal dense core vesicle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.

蛋白家族:

Belongs to the NPY family.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

文献引用

1). Metabolic regulation of Ganoderma lucidum extracts in high sugar and fat diet-induced obese mice by regulating the gut-brain axis. Journal of Functional Foods, 2020 [IF=5.6]
2). Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism. Molecular Medicine Reports, 2020 [IF=3.4]

Application: WB    Species: Mouse    Sample:

Figure 1. - Expression of NPY mRNA and protein in MC3T3-E1 cells. (A) Effects of siRNA inhibition on the expression of NPY mRNA at 48 h. **P
3). Chronic heat stress alters hypothalamus integrity, the serum indexes and attenuates expressions of hypothalamic appetite genes in broilers. Journal of Thermal Biology, 2019 (PubMed: 30975407) [IF=2.7]

Application: IF/ICC    Species: broilers    Sample: hypothalamus

Fig. 5. |Effect of chronic heat stress on protein levels of NPY in the hypothalamus of broilers after 14 days heat stress based on the whole-brain immunofluorescent method of the control group (A), heat stress group (B), pair-fed group (C). NPY, neuropeptide Y. White arrows on the right row panels indicates NPY proteins which colors are red.
4). Electroacupuncture alleviates Parkinson disease and regulates the expression of brain-gut peptides. EXPERIMENTAL ANIMALS, 2020 (PubMed: 32669479) [IF=2.4]

Application: WB    Species: rat    Sample: brain

Fig. 5.| Effects of EA on the levels of brain-gut peptides. A. The expression of NPY, CCK, and SST in the brain substantia nigra of rats from NC group, Sham group, 6-OHDA group, and 6-OHDA+EA group was determined by Western blot. B. The levels of NPY,CCK, SST, MTL, GAS, and PYY in the serum of rats from each group were detected by ELSA. Data are expressed as mean ±SD. *P<0.05, **P<0.01.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.