产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6437, RRID:AB_2838400.
展开/折叠
Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H quinone dehydrogenase 1; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1; NQO1_HUMAN; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1;
抗原和靶标
- P15559 NQO1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15559 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S13 | Phosphorylation | Uniprot | |
Y20 | Phosphorylation | Uniprot | |
K23 | Ubiquitination | Uniprot | |
K31 | Acetylation | Uniprot | |
K31 | Ubiquitination | Uniprot | |
K33 | Ubiquitination | Uniprot | |
S40 | Phosphorylation | Uniprot | |
Y43 | Phosphorylation | Uniprot | |
K54 | Ubiquitination | Uniprot | |
K59 | Acetylation | Uniprot | |
K59 | Ubiquitination | Uniprot | |
K61 | Ubiquitination | Uniprot | |
Y68 | Phosphorylation | Uniprot | |
Y76 | Phosphorylation | Uniprot | |
K77 | Acetylation | Uniprot | |
K77 | Ubiquitination | Uniprot | |
S82 | Phosphorylation | Uniprot | |
K90 | Ubiquitination | Uniprot | |
K91 | Ubiquitination | Uniprot | |
Y127 | Phosphorylation | Uniprot | |
Y129 | Phosphorylation | Uniprot | |
Y133 | Phosphorylation | Uniprot | |
K135 | Ubiquitination | Uniprot | |
K209 | Acetylation | Uniprot | |
K209 | Ubiquitination | Uniprot | |
K210 | Acetylation | Uniprot | |
K210 | Ubiquitination | Uniprot | |
K241 | Ubiquitination | Uniprot | |
K248 | Ubiquitination | Uniprot | |
K251 | Sumoylation | Uniprot | |
K251 | Ubiquitination | Uniprot | |
S255 | Phosphorylation | Uniprot | |
K262 | Acetylation | Uniprot | |
K262 | Ubiquitination | Uniprot | |
K271 | Sumoylation | Uniprot | |
K271 | Ubiquitination | Uniprot |
研究背景
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Cytoplasm.
Homodimer. Interacts with PDLIM4 isoform 2; this interaction stabilizes PDLIM4 isoform 2 in response to oxidative stress and protects it from ubiquitin-independent degradation by the core 20S proteasome.
Belongs to the NAD(P)H dehydrogenase (quinone) family.
研究领域
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Metabolism > Metabolism of cofactors and vitamins > Ubiquinone and other terpenoid-quinone biosynthesis.
文献引用
Application: WB Species: Rat Sample: PC-12 cells
Application: WB Species: mouse Sample: kidney
Application: WB Species: Rat Sample: PC12 cells
Application: IHC Species: Human Sample: HCC cell
Application: WB Species: mouse Sample: Hippocampi
Application: WB Species: Rat Sample:
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