产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6444, RRID:AB_2838407.
展开/折叠
G6PD; G6PD_HUMAN; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; Glucose 6 phosphate dehydrogenase; Glucose 6 phosphate dehydrogenase, G6PD; Glucose-6-phosphate 1-dehydrogenase; MET19; POS10; Zwf1p;
抗原和靶标
- P11413 G6PD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P11413 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
S8 | Phosphorylation | Uniprot | |
R9 | Methylation | Uniprot | |
T10 | Phosphorylation | Uniprot | |
T76 | Phosphorylation | Uniprot | |
K82 | Ubiquitination | Uniprot | |
K89 | Acetylation | Uniprot | |
K89 | Ubiquitination | Uniprot | |
K95 | Acetylation | Uniprot | |
K95 | Ubiquitination | Uniprot | |
K97 | Acetylation | Uniprot | |
K97 | Ubiquitination | Uniprot | |
S106 | Phosphorylation | Uniprot | |
Y107 | Phosphorylation | Uniprot | |
Y112 | Phosphorylation | Uniprot | |
S123 | Phosphorylation | Uniprot | |
S132 | Phosphorylation | Uniprot | |
Y139 | Phosphorylation | Uniprot | |
Y147 | Phosphorylation | Uniprot | |
K171 | Acetylation | Uniprot | |
K171 | Ubiquitination | Uniprot | |
S180 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
R182 | Methylation | Uniprot | |
Y202 | Phosphorylation | Uniprot | |
K205 | Ubiquitination | Uniprot | |
T236 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
Y249 | Phosphorylation | Uniprot | |
K293 | Ubiquitination | Uniprot | |
S296 | Phosphorylation | Uniprot | |
K320 | Ubiquitination | Uniprot | |
Y322 | Phosphorylation | Uniprot | |
K386 | Acetylation | Uniprot | |
K386 | Ubiquitination | Uniprot | |
Y401 | Phosphorylation | Uniprot | |
T402 | Phosphorylation | Uniprot | |
K403 | Acetylation | Uniprot | |
K403 | Ubiquitination | Uniprot | |
K408 | Acetylation | Uniprot | |
K408 | Ubiquitination | Uniprot | |
S418 | Phosphorylation | Uniprot | |
T423 | Phosphorylation | Uniprot | |
Y424 | Phosphorylation | Uniprot | |
Y428 | Phosphorylation | P12931 (SRC) | Uniprot |
K432 | Acetylation | Uniprot | |
K432 | Ubiquitination | Uniprot | |
S448 | Phosphorylation | Uniprot | |
T490 | Phosphorylation | Uniprot | |
K497 | Acetylation | Uniprot | |
K497 | Ubiquitination | Uniprot | |
Y503 | Phosphorylation | Uniprot | |
T506 | Phosphorylation | Uniprot | |
Y507 | Phosphorylation | P12931 (SRC) | Uniprot |
K514 | Acetylation | Uniprot | |
K514 | Ubiquitination | Uniprot |
研究背景
Cytosolic glucose-6-phosphate dehydrogenase that catalyzes the first and rate-limiting step of the oxidative branch within the pentose phosphate pathway/shunt, an alternative route to glycolysis for the dissimilation of carbohydrates and a major source of reducing power and metabolic intermediates for fatty acid and nucleic acid biosynthetic processes.
Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
Cytoplasm>Cytosol. Membrane>Peripheral membrane protein.
Isoform Long is found in lymphoblasts, granulocytes and sperm.
Homotetramer; dimer of dimers. Interacts with SIRT2; the interaction is enhanced by H(2)O(2) treatment.
Belongs to the glucose-6-phosphate dehydrogenase family.
研究领域
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.
· Metabolism > Metabolism of other amino acids > Glutathione metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
文献引用
Application: WB Species: rat Sample: cardiac tissues and H9c2 cells
Application: WB Species: mouse Sample: liver
Application: WB Species: Human Sample: HepG2 cells
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