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| 产品: | CDK6 抗体 |
| 货号: | DF6448 |
| 描述: | Rabbit polyclonal antibody to CDK6 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken |
| 分子量: | 36kDa; 37kD(Calculated). |
| 蛋白号: | Q00534 |
| RRID: | AB_2838411 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6448, RRID:AB_2838411.
展开/折叠
CDK 6; CDK6; CDK6_HUMAN; Cell division protein kinase 6; Crk 2; Crk2; Cyclin dependent kinase 6; Cyclin-dependent kinase 6; MCPH12; MGC59692; p40; PLSTIRE; Serine/threonine protein kinase PLSTIRE; Serine/threonine-protein kinase PLSTIRE; STQTL11;
抗原和靶标
Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.
- Q00534 CDK6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q00534 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K3 | Acetylation | Uniprot | |
| K3 | Methylation | Uniprot | |
| K3 | Ubiquitination | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| Y24 | Phosphorylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K43 | Acetylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| T177 | Phosphorylation | P50613 (CDK7) | Uniprot |
| S222 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K264 | Acetylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| T267 | Phosphorylation | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| Y299 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot |
翻译修饰 - Q00534 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P06400 (RB1) | S612 | Uniprot |
| P06400 (RB1) | S780 | Uniprot |
| P06400 (RB1) | S788 | Uniprot |
| P06400 (RB1) | S795 | Uniprot |
| P06400 (RB1) | S807 | Uniprot |
| P06400 (RB1) | S811 | Uniprot |
| P06400 (RB1) | T821 | Uniprot |
| P06400 (RB1) | T826 | Uniprot |
| P06748 (NPM1) | T199 | Uniprot |
| P10415 (BCL2) | S70 | Uniprot |
| P10415 (BCL2) | S87 | Uniprot |
| P35222 (CTNNB1) | S45 | Uniprot |
| P38936 (CDKN1A) | S130 | Uniprot |
| P46527 (CDKN1B) | S10 | Uniprot |
| P46527 (CDKN1B) | T187 | Uniprot |
| Q01196 (RUNX1) | S21 | Uniprot |
| Q01196 (RUNX1) | S249 | Uniprot |
| Q01196 (RUNX1) | S266 | Uniprot |
| Q01196 (RUNX1) | T273 | Uniprot |
| Q01196 (RUNX1) | S276 | Uniprot |
| Q01196-8 (RUNX1) | S293 | Uniprot |
| Q01196-8 (RUNX1) | T300 | Uniprot |
| Q01196-8 (RUNX1) | S303 | Uniprot |
| Q01196 (RUNX1) | S397 | Uniprot |
| Q04206 (RELA) | S536 | Uniprot |
| Q05682 (CALD1) | T730 | Uniprot |
| Q05682 (CALD1) | S789 | Uniprot |
| Q06830 (PRDX1) | T90 | Uniprot |
| Q08050 (FOXM1) | S4 | Uniprot |
| Q08050 (FOXM1) | S35 | Uniprot |
| Q08050 (FOXM1) | S451 | Uniprot |
| Q08050 (FOXM1) | S489 | Uniprot |
| Q08050 (FOXM1) | S508 | Uniprot |
| Q08050 (FOXM1) | T510 | Uniprot |
| Q08050 (FOXM1) | S522 | Uniprot |
| Q08050 (FOXM1) | T600 | Uniprot |
| Q08050 (FOXM1) | T611 | Uniprot |
| Q08050 (FOXM1) | T620 | Uniprot |
| Q08050 (FOXM1) | T627 | Uniprot |
| Q08050 (FOXM1) | S704 | Uniprot |
| Q08999 (RBL2) | T401 | Uniprot |
| Q08999 (RBL2) | S672 | Uniprot |
| Q08999 (RBL2) | S1035 | Uniprot |
| Q92879 (CELF1) | S302 | Uniprot |
| Q96KS0 (EGLN2) | S130 | Uniprot |
研究背景
Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases.
Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.
Cytoplasm. Nucleus. Cell projection>Ruffle. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663).
Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.
Interaction with D-type G1 cyclins. Cyclin binding promotes enzyme activation by phosphorylation at Thr-177 (By similarity). Binds to RUNX1, CDKN2D, FBXO7 and CDKN2C/p18-INK4c. Forms a cytoplasmic complex with Hsp90/HSP90AB1 and CDC37. FBXO7-binding promotes D-type cyclin binding. Interacts with Kaposi's sarcoma herpesvirus (KSHV) V-cyclin and herpesvirus saimiri (V-cyclin/ECLF2); the CDK6/V-cyclin complex phosphorylates NPM1 and thus lead to viral reactivation by reducing viral LANA levels.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Glioma. (View pathway)
· Human Diseases > Cancers: Specific types > Melanoma. (View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
文献引用
Application: WB Species: Human Sample: HO8910 and A2780 cells
Application: WB Species: human Sample:
Application: WB Species: human Sample: human
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